Imaging pancreatic cancer using surface-functionalized quantum dots

被引：90

作者：

Qian, Jun

Yong, Ken-Tye

Roy, Indrajit

Ohulchanskyy, Tymish Y.

Bergey, Earl J.

Lee, Hoon Hi

Tramposch, Kenneth M.

He, Sailing

Maitra, Anirban

Prasad, Paras N. ^{[1
]}

机构：

[1] SUNY Buffalo, Inst Lasers Photon & Biophoton, Buffalo, NY 14260 USA

[2] Zhejiang Univ, Ctr Opt & Electromagnet Res, Hangzhou 310058, Peoples R China

[3] Johns Hopkins Univ, Sch Med, Sol Goldman Pancreat Canc Res Ctr, Dept Pathol & Oncol, Baltimore, MD 21231 USA

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2007年 / 111卷 / 25期

关键词：

GROWTH;

D O I：

10.1021/jp070620n

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In this study, CdSe/CdS/ZnS quantum dots (QDs) were used as optical contrast agent for imaging pancreatic cancer cells in vitro using transferrin and anti-Claudin-4 as targeting ligands. CdSe/CdS/ZnS was chosen because the CdSe/CdS/ZnS QDs have better photoluminescence (PL) efficiency and stability than those of CdSe/ZnS. The transferrin-mediated targeting is demonstrated in both a cell-free coprecipitation assay as well as using in vitro confocal microscopy. Pancreatic cancer specific uptake is also demonstrated using the monoclonal antibody anti-Claudin-4. This targeted QD platform will be further modified for the purpose of developing as an early detection imaging tool for pancreatic cancer.

引用

收藏

页码：6969 / 6972

页数：4

相关论文

共 17 条

[1] Semiconductor nanocrystals as fluorescent biological labels [J].

Bruchez, M ;

Moronne, M ;

Gin, P ;

Weiss, S ;

Alivisatos, AP .

SCIENCE, 1998, 281 (5385) :2013-2016

[2] Identifying allelic loss and homozygous deletions in pancreatic cancer without matched normals using high-density single-nucleotide polymorphism arrays [J].

Calhoun, Eric S. ;

Hucl, Tomas ;

Gallmeier, Eike ;

West, Kristen M. ;

Arking, Dan E. ;

Maitra, Anirban ;

Iacobuzio-Donahue, Christine A. ;

Chakravarti, Aravinda ;

Hruban, Ralph H. ;

Kern, Scott E. .

CANCER RESEARCH, 2006, 66 (16) :7920-7928

[3] Preinvasive and invasive ductal pancreatic cancer and its early detection in the mouse [J].

Hingorani, SR ;

Petricoin, EF ;

Maitra, A ;

Rajapakse, V ;

King, C ;

Jacobetz, MA ;

Ross, S ;

Conrads, TP ;

Veenstra, TD ;

Hitt, BA ;

Kawaguchi, Y ;

Johann, D ;

Liotta, LA ;

Crawford, HC ;

Putt, ME ;

Jacks, T ;

Wright, CVE ;

Hruban, RH ;

Lowy, AM ;

Tuveson, DA .

CANCER CELL, 2003, 4 (06) :437-450

[4] Design and characterization of lysine cross-linked mereapto-acid biocompatible quantum dots [J].

Jiang, W ;

Mardyani, S ;

Fischer, H ;

Chan, WCW .

CHEMISTRY OF MATERIALS, 2006, 18 (04) :872-878

[5] Optimizing the synthesis of red- to near-IR-emitting CdS-capped CdTexSe1-x alloyed quantum dots for biomedical imaging [J].

Jiang, Wen ;

Singhal, Anupam ;

Zheng, Jianing ;

Wang, Chen ;

Chan, Warren C. W. .

CHEMISTRY OF MATERIALS, 2006, 18 (20) :4845-4854

[6] Epitaxial growth and photochemical annealing of graded CdS/ZnS shells on colloidal CdSe nanorods [J].

Manna, L ;

Scher, EC ;

Li, LS ;

Alivisatos, AP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (24) :7136-7145

[7] Synthesis of high quality zinc blende CdSe nanocrystals [J].

Mohamed, MB ;

Tonti, D ;

Al-Salman, A ;

Chemseddine, A ;

Chergui, M .

JOURNAL OF PHYSICAL CHEMISTRY B, 2005, 109 (21) :10533-10537

[8] Claudin 4 protein expression in primary and metastatic pancreatic cancer - Support for use as a therapeutic target [J].

Nichols, LS ;

Ashfaq, R ;

Iacobuzio-Donahue, CA .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2004, 121 (02) :226-230

[9]

Prasad P. N., 2004, NANOPHOTONICS

[10]

PRASAD PN, 2004, BIOPHOTONICS