Cloning and functional expression of cycloartenol synthases from mangrove species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce

被引：21

作者：

Basyuni, Mohammad

Oku, Hirosuke ^{[1
]}

Tsujimoto, Etsuko

Baba, Shigeyuki

机构：

[1] Univ Ryukyus, Ctr Mol Biosci, Div Mol Biotechnol, Nishihara, Okinawa 9030213, Japan

[2] Kagoshima Univ, United Grad Sch Agr Sci, Kagoshima 8900065, Japan

[3] Univ Ryukyus, Fac Agr, Grad Sch Agr, Nishihara, Okinawa 9030213, Japan

[4] Univ Ryukyus, Trop Biosphere Res Ctr, Nishihara, Okinawa 9030213, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2007年 / 71卷 / 07期

关键词：

cycloartenol synthase; Kandelia candel (L.) Druce; mangrove; oxidosqualene cyclase; Rhizophora stylosa Griff;

D O I：

10.1271/bbb.70113

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To obtain cDNAs encoding oxidosqualene cyclase (OSC), we cloned two cDNAs, KcCAS and RsCAS, from roots of Kandelia candel (L.) Druce and leaves of Rhizophora stylosa Griff. by homology based PCR method respectively. The deduced amino acid sequences of both OSCs showed 82% homology to cycloartenol synthases from Lotus japonicus (OSC5) and Ricinus cummunis (RcCAS), suggesting that these are cycloartenol synthases of K. candel and R. stylosa; The genes obtained were expressed in a lanosterol synthase deficient Saccharomyces cerevisiae (ERG7) strain, GIL77. GC-MS analysis identified the accumulated reaction product in the yeast transformant to be cycloartenol, indicating that both KcCAS and RsCAS encode cycloartenol synthase.

引用

页码：1788 / 1792

页数：5

共 28 条

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