Loss of AtPIN1 does not influence the in vitro morphogenic potential of Arabidopsis thaliana suspension cultures

被引：1

作者：

Eneva, T ^{[1
]}

Tietz, O ^{[1
]}

Luley, E ^{[1
]}

Palme, K ^{[1
]}

机构：

[1] Univ Freiburg, Inst Biol 2, D-79104 Freiburg, Germany

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2004年 / 79卷 / 02期

关键词：

AtPIN1; mutant; auxin depletion; long-term suspension culture; shoot regeneration;

D O I：

10.1007/s11240-004-0658-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Suspension cultures from Arabidopsis thaliana wild type and AtPIN1-deficient lines were initiated and maintained for more than 3 years. A protocol for efficient regeneration from long-term suspension cultures was established. Arabidopsis wild-type and respectively AtPIN1 mutant plants have been regenerated from these cultures and characterized. Additionally, transgenic suspension cultures expressing the uidA (beta-glucuronidase) reporter gene under the control of AtPIN1 promoter have been used for morphogenic studies. Our studies suggest that a lack of AtPIN1 function affects shoot differentiation and development, but does not influence in vitro regeneration of plants.

引用

页码：181 / 188

页数：8

共 23 条

[1] Local, efflux-dependent auxin gradients as a common module for plant organ formation [J].