TIF-IA, the factor mediating growth-dependent control of ribosomal RNA synthesis, is the mammalian homolog of yeast Rrn3p

被引：110

作者：

Bodem, J ^{[1
]}

Dobreva, G ^{[1
]}

Hoffman-Rohrer, U ^{[1
]}

Iben, S ^{[1
]}

Zentgraf, H ^{[1
]}

Delius, H ^{[1
]}

Vingron, M ^{[1
]}

Grummt, I ^{[1
]}

机构：

[1] German Canc Res Ctr, Div Mol Biol Cell 2, D-69120 Heidelberg, Germany

来源：

EMBO REPORTS | 2000年 / 1卷 / 02期

关键词：

D O I：

10.1093/embo-reports/kvd032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cells carefully modulate the rate of rRNA transcription in order to prevent an overinvestment in ribosome synthesis under less favorable nutritional conditions. In mammals, growth-dependent regulation of RNA polymerase I (Pol I) transcription is mediated by TIF-IA, an essential initiation factor that is active in extracts from growing but not starved or cycloheximide-treated mammalian cells. Here we report the molecular cloning and functional characterization of recombinant TIF-IA, which turns out to be the mammalian homolog of the yeast factor Rrn3p. We demonstrate that TIF-IA interacts with Pol I in the absence of template DNA, augments Pol I transcription in vivo and rescues transcription in extracts from growth-arrested cells in vitro.

引用

页码：171 / 175

页数：5

共 13 条

[1] FACTOR-C, THE SPECIFIC INITIATION COMPONENT OF THE MOUSE RNA-POLYMERASE-I HOLOENZYME, IS INACTIVATED EARLY IN THE TRANSCRIPTION PROCESS [J].

BRUN, RP ;

RYAN, K ;

SOLLNERWEBB, B .

MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (07) :5010-5021

[2] p53 represses ribosomal gene transcription [J].

Budde, A ;

Grummt, I .

ONCOGENE, 1999, 18 (04) :1119-1124

[3] GROWTH-DEPENDENT REGULATION OF RIBOSOMAL-RNA SYNTHESIS IS MEDIATED BY A TRANSCRIPTION INITIATION-FACTOR (TIF-IA) [J].

BUTTGEREIT, D ;

PFLUGFELDER, G ;

GRUMMT, I .

NUCLEIC ACIDS RESEARCH, 1985, 13 (22) :8165-8180

[4]

Grummt I, 1999, PROG NUCLEIC ACID RE, V62, P109

[5]

MAHAJAN PB, 1990, J BIOL CHEM, V265, P16225

[6] Specialized form of RNA polymerase I, essential for initiation and growth-dependent regulation of rRNA synthesis, is disrupted during transcription [J].

Milkereit, P ;

Tschochner, H .

EMBO JOURNAL, 1998, 17 (13) :3692-3703

[7] RNA polymerase I transcription factor Rrn3 is functionally conserved between yeast and human [J].

Moorefield, B ;

Greene, EA ;

Reeder, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (09) :4724-4729

[8]

Schnapp A, 1996, METHOD ENZYMOL, V273, P233

[9] A GROWTH-DEPENDENT TRANSCRIPTION INITIATION-FACTOR (TIF-IA) INTERACTING WITH RNA POLYMERASE-I REGULATES MOUSE RIBOSOMAL-RNA SYNTHESIS [J].

SCHNAPP, A ;

PFLEIDERER, C ;

ROSENBAUER, H ;

GRUMMT, I .

EMBO JOURNAL, 1990, 9 (09) :2857-2863

[10] FUNCTION OF THE GROWTH-REGULATED TRANSCRIPTION INITIATION FACTOR-TIF-IA IN INITIATION COMPLEX-FORMATION AT THE MURINE RIBOSOMAL GENE PROMOTER [J].

SCHNAPP, A ;

SCHNAPP, G ;

ERNY, B ;

GRUMMT, I .

MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (11) :6723-6732

← 1 2 →