Errors in the measurement of cross-correlated relaxation rates and how to avoid them

被引:14
作者
Carlomagno, T [1 ]
Griesinger, C [1 ]
机构
[1] Univ Frankfurt, Inst Organ Chem, D-60439 Frankfurt, Germany
关键词
cross-correlated relaxation; constant time; systematic errors; J-resolved Gamma experiment; quantitative Gamma experiment;
D O I
10.1006/jmre.2000.2056
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cross-correlated relaxation rates Gamma are commonly obtained from constant time experiments by measuring the effect of the desired cross-correlated relaxation on an appropriate coherence during the constant time T. These measurements are affected by systematic errors, which derive from undesired cross-correlated relaxation effects taking place before and after the constant time period T. In this paper we discuss the sources and the size of these errors in an example of two pulse sequences. Higher accuracy of the measured data can be obtained by recording a set of experiments with different T values. Cross-correlated relaxation rates me measured in constant time experiments either from the differential relaxation of multiplet components (J-resolved Gamma experiments) or from the efficiency of magnetization transfer between two coherences (quantitative Gamma experiments). In this paper we calculate analytically the statistical errors in both J-resolved and quantitative Gamma experiments. These formulae provide the basis for the choice of the most efficient experimental approach and parameters for a given measurement time and size of the rate. The optimal constant time T for each method can be calculated and depends on the relaxation properties of the molecule under investigation. Moreover, we will show how to optimize the relative duration of cross and reference experiments in a quantitative Gamma approach. (C) 2000 Academic Press.
引用
收藏
页码:280 / 287
页数:8
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