Distinct subsets of microRNAs are expressed differentially in the human placentas of patients with preeclampsia

被引：441

作者：

Pineles, Beth L. ^{[1
]}

Romero, Roberto

Montenegro, Daniel

Tarca, Adi L.

Han, Yu Mi

Kim, Yeon Mee

Draghici, Sorin

Espinoza, Jimmy

Kusanovic, Juan Pedro

Mittal, Pooja

Hassan, Sonia S.

Kim, Chong Jai

机构：

[1] NICHHD, Perinatol Res Branch, Dept Hlth & Human Serv, NIH, Bethesda, MD 20892 USA

[2] NICHHD, Perinatol Res Branch, Dept Hlth & Human Serv, NIH, Detroit, MI USA

[3] Wayne State Univ, Ctr Mol Med & Genet, Detroit, MI USA

[4] Wayne State Univ, Dept Comp Sci, Detroit, MI 48202 USA

[5] Wayne State Univ, Sch Med, Dept Pathol, Detroit, MI 48201 USA

[6] Wayne State Univ, Sch Med, Dept Obstet & Gynecol, Detroit, MI 48201 USA

来源：

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | 2007年 / 196卷 / 03期

基金：

美国国家卫生研究院;

关键词：

microRNA; placenta; preeclampsia; small-for-gestational age;

D O I：

10.1016/j.ajog.2007.01.008

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: Preeclampsia and small-for-gestational age (SGA) neonates have partially overlapping clinicopathologic features. MicroRNAs (miRNAs) are critical posttranscriptional regulators of gene expression. This study was performed to determine whether preeclampsia and SGA are associated with alterations in placental miRNA expression. Study design: Placentas were obtained from patients with (1) preeclampsia (n = 9); (2) SGA (n = 9); (3) preeclampsia + SGA (n = 9); and (4) a control group with spontaneous preterm labor and delivery (PTL; n = 9). The expression of 157 miRNAs was assessed by real-time quantitative reverse transcription-polymerase chain reaction. Results: Differential expression between preeclampsia and the control group (miR-210, miR-182) and between preeclampsia + SGA and the control group (miR-210, miR-182*, and others) was found. Gene Ontology analysis of the target genes revealed enrichment for specific biological process categories (antiapoptosis: miR-182; regulation of transcription: miR-210). Conclusion: This study reports, for the first time, increased expression of specific placental miRNAs in preeclampsia with and without SGA. The findings also provide novel targets for further investigation of the pathophysiology of preeclampsia. © 2007 Mosby, Inc. All rights reserved.

引用

页码：261 / 263

页数：3

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