Isolation of microsatellite loci in artichoke (Cynara cardunculus L. var. scolymus)

被引：41

作者：

Acquadro, A ^{[1
]}

Portis, E ^{[1
]}

Lanteri, S ^{[1
]}

机构：

[1] Univ Turin, DiVaPRA Plant Genet & Breeding, I-10095 Turin, Italy

来源：

MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 01期

关键词：

artichoke; Cynara cardunculus L. var. scolymus; database search; enriched library; SSRs;

D O I：

10.1046/j.1471-8286.2003.00343.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the development of nine microsatellite markers in globe artichoke (Cynara cardunculus L. var. scolymus). Four markers were obtained from sequences available in GenBank and five were isolated using a biotin/streptavidin capture technique for (CA)(n) and (CT)(n) motifs directly from artichoke genomic DNA. Polymorphism was explored in 15 artichoke accessions that represent the genetic variation within cultivated varietal types. Inter-specific amplification was tested using cultivated cardoon (C. cardunculus L. var. altilis D.C.) and wild cardoon (C. cardunculus L. var. sylvestris Lam.). Primers and conditions for polymerase chain reaction (PCR) amplification of detected loci are described.

引用

页码：37 / 39

页数：3

共 10 条

[1]

Elia A., 1996, Advances in Horticultural Science, V10, P158

[2] RAPD variation within and among populations of globe artichoke cultivar 'Spinoso sardo' [J].