White spot syndrome virus (WSSV) in cultured Penaeus monodon in the Philippines

被引：46

作者：

Magbanua, FO

Natividad, KT

Migo, VP

Alfafara, CG

de la Peña, FO

Miranda, RO

Albaladejo, JD

Nadala, ECB

Loh, PC

Mahilum-Tapay, L ^{[1
]}

机构：

[1] Univ Philippines, Los Banos Coll, Natl Inst Mol Biol & Biotechnol, Laguna 4031, Philippines

[2] Bur Fisheries & Aquat Resources, Dept Agr, Quezon City 1001, Philippines

[3] Univ Cambridge, Dept Haematol, Cambridge CB2 2PT, England

[4] Univ Hawaii, Dept Microbiol, Virus Lab, Honolulu, HI 96822 USA

来源：

DISEASES OF AQUATIC ORGANISMS | 2000年 / 42卷 / 01期

关键词：

white spot syndrome virus; polymerase chain reaction; Western blot;

D O I：

10.3354/dao042077

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The prevalence and geographic distribution of white spot syndrome virus (WSSV) infection among cultured penaeid shrimp in the Philippines was determined from January to May, 1999, using PCR (polymerase chain reaction) protocol and Western blot assays. A total of 71 samples consisting of 18 post-larvae (PL) and 53 juvenile/adult shrimp samples (56 to 150 days-of-culture, DOG) were screened for WSSV. Of the 71 samples tested, 51 (72%) were found positive for WSSV by PCR: 61% (31/51) after 1-step PCR and 39% (20/51) after a-step, non-nested PCR. Of the PL and juvenile/adult shrimp samples tested, 50 and 79% were positive for WSSV, respectively. By Western blot, only 6 of the 51 (12%) PCR-positive samples tested positive for WSSV. Of the 20 samples negative for WSSV by PCR, all tested negative for WSSV by Western blot assay. This is the first report of the occurrence of WSSV in the Philippines.

引用

页码：77 / 82

页数：6

共 22 条

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ALBALADEJO JD, 1998, ADV SHRIMP BIOTECHNO, P251

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BELAK J, 1999, P AC CONS MAR SHRIMP

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