Investigation of the translation-initiation factor IF2 gene, infB, as a tool to study the population structure of Streptococcus agalactiae

被引:17
作者
Hedegaard, J
Hauge, M
Fage-Larsen, J
Mortensen, KK
Kilian, M
Sperling-Petersen, HU
Poulsen, K
机构
[1] Aarhus Univ, Dept Med Microbiol & Immunol, DK-8000 Aarhus C, Denmark
[2] Aarhus Univ, Inst Mol & Struct Biol, Dept Biostruct Chem, DK-8000 Aarhus, Denmark
来源
MICROBIOLOGY-UK | 2000年 / 146卷
关键词
Streptococcus agalactiae; infB; initiation factor 2; clonal population structure;
D O I
10.1099/00221287-146-7-1661
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The sequence of infB, encoding the prokaryotic translation-initiation factor 2 (IF2), was determined in eight strains of Streptococcus agalactiae (group B streptococcus) and an alignment revealed limited intraspecies diversity within S. agalactiae. The amino acid sequence of IF2 from S. agalactiae and from related species were aligned and revealed an interspecies conserved central and C-terminal part, and an N-terminal part that is highly variable in length and amino acid sequence. The diversity and relationships in a collection of 58 genetically distinct strains of S. agalactiae were evaluated by comparing a partial sequence of infB. A total of six alleles were detected for the region of infB analysed. The alleles correlated with the separation of the same strains of S. agalactiae into major evolutionary lineages, as shown in previous work. The partial sequences of infB were furthermore used in phylogenetic analyses of species closely related to S. agalactiae, yielding an evolutionary tree which had a topology similar to a tree constructed using 16S rRNA sequences from the same species.
引用
收藏
页码:1661 / 1670
页数:10
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