Regions of human chromosome 2 (2q32-q35) and mouse chromosome 1 show synteny with the pufferfish genome (Fugu rubripes)

被引：21

作者：

Schofield, JP ^{[1
]}

Elgar, G ^{[1
]}

Greystrong, J ^{[1
]}

Lye, G ^{[1
]}

Deadman, R ^{[1
]}

Micklem, G ^{[1
]}

King, A ^{[1
]}

Brenner, S ^{[1
]}

Vaudin, M ^{[1
]}

机构：

[1] SANGER CTR,CAMBRIDGE CB10 1SA,ENGLAND

来源：

GENOMICS | 1997年 / 45卷 / 01期

基金：

英国惠康基金;

关键词：

D O I：

10.1006/geno.1997.4913

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have isolated and sequenced a cosmid clone from the compact genome of the Japanese pufferfish (Fugu rubripes) containing portions of three genes that have the same order as in human. The gene order is microtubule-associated protein (MAP-2), myosin Light chain (MYL-1), and carbamoyl phosphate synthetase (CPS III). The intron-exon organization of Fugu CPS III is identical with that of rat CPS I, although the equivalent genomic fragments of rat and Fugu CPS span 87.9 and 21 kb, respectively. This is the first report of a piscine CPS III genomic structure and predicts a close evolutionary link between CPS III and CPS I. The 8-kb intergenic region between MYL-1 and CPS gave no clear areas of transcription factor-binding sites by pairwise comparison with shark or rat CPS promoter regions. However, there was a match with the rat myosin light chain 2 (MLC-S) gene promoter and a MyoD transcription factor-binding site 874 bp upstream of the MYL-1 gene. (C) 1997 Academic Press.

引用

页码：158 / 167

页数：10

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