Thermodynamic and spectral properties of DNA miniduplexes with the terminal G•A mispairs and 3′/or 5′ dangling bases

被引：39

作者：

Lokhov, SG ^{[1
]}

Pyshnyi, DV ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Bioorgan Chem, Siberian Div, Novosibirsk 630090, Russia

来源：

FEBS LETTERS | 1997年 / 420卷 / 2-3期

关键词：

deoxyriboduplex; thermodynamics; GA mismatch; dangling base;

D O I：

10.1016/S0014-5793(97)01502-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tetradeoxyribonucleotide pAGCG in 1 M NaCl forms duplexes with terminal non-canonical pA.G pairs with stability significantly exceeding that for the duplex (pAGCT)(2) and lower than that for the duplex (pCGCG)(2). The deosyriboduplex (pAGCG)(2) is considerably stabilized by 3'-Y and slightly bg 5'-X dangling bases, Therefore, the stability of duplexes with 3' dangling bases decreases in the order (pAGCGY)(2) > (pCGCGA)(2) > (pAGCTA)(2). The sum of the independent stabilizing effect of the of 5'-pG and 3'-A dangling bases on the (pAGCG)(2) core duplex is higher than that of the additional terminal pG.A pair in pG-A-/-G-A tandem of the duplex (pGAGCGA)(2). (C) 1997 Federation of European Biochemical Societies.

引用

页码：134 / 138

页数：5

共 28 条

[1] SYNTHESIS AND CHARACTERIZATION OF OLIGODEOXYRIBONUCLEOTIDES CONTAINING TERMINAL PHOSPHATES - NMR, UV SPECTROSCOPIC AND THERMODYNAMIC ANALYSIS OF DUPLEX FORMATION OF [D(PGGAATTCC)]2, [D(GGAATTCCP)]2 AND [D(PGGAATTCCP)]2 [J].

BOWER, M ;

SUMMERS, MF ;

KELL, B ;

HOSKINS, J ;

ZON, G ;

WILSON, WD .

NUCLEIC ACIDS RESEARCH, 1987, 15 (08) :3531-3547

[2] STRUCTURAL POLYMORPHISM OF D(GA.TC)(N) DNA-SEQUENCES - INTRAMOLECULAR AND INTERMOLECULAR ASSOCIATIONS OF THE INDIVIDUAL STRANDS [J].

CASASNOVAS, JM ;

HUERTAS, D ;

ORTIZLOMBARDIA, M ;

KYPR, J ;

AZORIN, F .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 233 (04) :671-681

[3] AN OLIGODEOXYRIBONUCLEOTIDE THAT SUPPORTS CATALYTIC ACTIVITY IN THE HAMMERHEAD RIBOZYME DOMAIN [J].

CHARTRAND, P ;

HARVEY, SC ;

FERBEYRE, G ;

USMAN, N ;

CEDERGREN, R .

NUCLEIC ACIDS RESEARCH, 1995, 23 (20) :4092-4096

[4] DNA-SEQUENCE GCGAATGAGC CONTAINING THE HUMAN CENTROMERE CORE SEQUENCE GAAT FORMS A SELF-COMPLEMENTARY DUPLEX WITH SHEARED G-CENTER-DOT-A PAIRS IN SOLUTION [J].

CHOU, SH ;

CHENG, JW ;

FEDOROFF, O ;

REID, BR .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 241 (03) :467-479

[5] VERY STABLE MISMATCH DUPLEXES - STRUCTURAL AND THERMODYNAMIC STUDIES ON TANDEM G.A MISMATCHES IN DNA [J].

EBEL, S ;

LANE, AN ;

BROWN, T .

BIOCHEMISTRY, 1992, 31 (48) :12083-12086

[6] IMPROVED FREE-ENERGY PARAMETERS FOR PREDICTIONS OF RNA DUPLEX STABILITY [J].

FREIER, SM ;

KIERZEK, R ;

JAEGER, JA ;

SUGIMOTO, N ;

CARUTHERS, MH ;

NEILSON, T ;

TURNER, DH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (24) :9373-9377

[7] SOLUTION STRUCTURE OF A GA MISMATCH DNA-SEQUENCE, D(CCATGAATGG)(2), DETERMINED BY 2D NMR AND STRUCTURAL REFINEMENT METHODS [J].

GREENE, KL ;

JONES, RL ;

LI, Y ;

ROBINSON, H ;

WANG, AHJ ;

ZON, G ;

WILSON, WD .

BIOCHEMISTRY, 1994, 33 (05) :1053-1062

[8] SOLVENT EFFECTS ON THE STABILITY OF A7U7P [J].

HICKEY, DR ;

TURNER, DH .

BIOCHEMISTRY, 1985, 24 (08) :2086-2094

[9] MOST COMPACT HAIRPIN-TURN STRUCTURE EXERTED BY A SHORT DNA FRAGMENT, D(GCGAAGC) IN SOLUTION - AN EXTRAORDINARILY STABLE STRUCTURE RESISTANT TO NUCLEASES AND HEAT [J].

HIRAO, I ;

KAWAI, G ;

YOSHIZAWA, S ;

NISHIMURA, Y ;

ISHIDO, Y ;

WATANABE, K ;

MIURA, K .

NUCLEIC ACIDS RESEARCH, 1994, 22 (04) :576-582

[10] ALTERNATING D(GA)(N) DNA-SEQUENCES FORM ANTIPARALLEL STRANDED HOMODUPLEXES STABILIZED BY THE FORMATION OF G.A BASE-PAIRS [J].

HUERTAS, D ;

BELLSOLELL, L ;

CASASNOVAS, JM ;

COLL, M ;

AZORIN, F .

EMBO JOURNAL, 1993, 12 (10) :4029-4038

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