Thermodynamic and spectral properties of DNA miniduplexes with the terminal G•A mispairs and 3′/or 5′ dangling bases

被引：40

作者：

Lokhov, SG ^{[1
]}

Pyshnyi, DV ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Bioorgan Chem, Siberian Div, Novosibirsk 630090, Russia

来源：

FEBS LETTERS | 1997年 / 420卷 / 2-3期

关键词：

deoxyriboduplex; thermodynamics; GA mismatch; dangling base;

D O I：

10.1016/S0014-5793(97)01502-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tetradeoxyribonucleotide pAGCG in 1 M NaCl forms duplexes with terminal non-canonical pA.G pairs with stability significantly exceeding that for the duplex (pAGCT)(2) and lower than that for the duplex (pCGCG)(2). The deosyriboduplex (pAGCG)(2) is considerably stabilized by 3'-Y and slightly bg 5'-X dangling bases, Therefore, the stability of duplexes with 3' dangling bases decreases in the order (pAGCGY)(2) > (pCGCGA)(2) > (pAGCTA)(2). The sum of the independent stabilizing effect of the of 5'-pG and 3'-A dangling bases on the (pAGCG)(2) core duplex is higher than that of the additional terminal pG.A pair in pG-A-/-G-A tandem of the duplex (pGAGCGA)(2). (C) 1997 Federation of European Biochemical Societies.

引用

页码：134 / 138

页数：5

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