Isocratic high-performance liquid chromatographic method for quantitative determination of lysine, histidine and tyrosine in foods

被引：29

作者：

Sanz, MA ^{[1
]}

Castillo, G ^{[1
]}

Hernandez, A ^{[1
]}

机构：

[1] UNIV ALCALA DE HENARES, DEPT NUTR & BROMATOL, E-28871 ALCALA DE HENARES, MADRID, SPAIN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 719卷 / 01期

关键词：

D O I：

10.1016/0021-9673(95)00232-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the quantitative determination of lysine, histidine and tyrosine in foods based on pre-column derivatization with 5-dimethylaminonaphthalene-1-sulfonyl chloride (DnsCl) and reversed-phase liquid chromatography has been developed. Derivatization conditions, including DnsCl concentration, time, temperature, and buffer solution were studied. To establish the reliability of the proposed liquid chromatographic (LC) method, the precision and accuracy of the analyses were evaluated using samples of casein and lysozyme.

引用

页码：195 / 201

页数：7

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