Differential effects of flagellins from Pseudomonas syringae pv. tabaci, tomato and glycinea on plant defense response

被引:54
作者
Taguchi, F [1 ]
Shimizu, R [1 ]
Nakajima, R [1 ]
Toyoda, K [1 ]
Shiraishi, T [1 ]
Ichinose, Y [1 ]
机构
[1] Okayama Univ, Fac Agr, Lab Plant Pathol & Genet Engn, Okayama 7008530, Japan
基金
日本学术振兴会;
关键词
cell death; elicitor; flagellin; FliC; hypersensitive reaction; Pseudomonas syringae; tobacco;
D O I
10.1016/S0981-9428(02)00018-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
To investigate the factor that determines incompatible interactions between Pseudomonas syringae pv. tabaci and non-host plants, an elicitor of hypersensitive reaction (HR) was partially purified from the supernatant of a nutrient-poor medium of bacterial culture by DEAE column chromatography. The major protein in the elicitor-active fractions was identified as a flagellin which is a component of flagellar filaments. The flagellins purified from P. syringae pv. tomato and glycinea, incompatible pathogens of tobacco plants, induced fragmentation of chromosomal DNA and oxidative burst accompanied by programmed cell death in tobacco (Nicotiana tabacum) Bright Yellow (BY-2) cells, but the flagellin from pv. tabaci, a compatible pathogen, did not. However, the amino acid sequences of flagellins deduced from fliC genes showed a high homology among these P. syringae pathovars. In particular, the amino acid sequences of pv. tabaci and glycinea are completely identical. However, both recombinant flagellins produced in Escherichia coli possess HR-inducing activity in BY-2 cells. These results indicate that the post-translational modification of flagellins has an important role for HR-inducing ability in tobacco cells. Furthermore, we discuss the cause of a different elicitor activity among flagellins on tobacco cells and the role of flagellins in the determining specificity. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
引用
收藏
页码:165 / 174
页数:10
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