An improved and rapid protocol for the isolation of poly(A)+-RNA from small samples of Scots pine seedlings

被引：2

作者：

Avila, C ^{[1
]}

Cánovas, FM ^{[1
]}

机构：

[1] Univ Malaga, Fac Ciencias, Lab Biol Mol & Bioquim, E-29071 Malaga, Spain

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 2000年 / 18卷 / 02期

关键词：

mRNA extraction; pine seedlings; RT-PCR application;

D O I：

10.1007/BF02824019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple rapid method for the isolation of poly(A)(+)-RNA from pine seedlings (Pinus sylvestris) was developed. The entire procedure can be carried out in Eppendorf tubes, thus greatly minimizing the time, starting material (as little as 3-5 mu g of total RNA) and the amount of chemicals required. The poly(A)(+)-RNA recovered is a suitable substrate for RT-PCR.

引用

页码：117 / 122

页数：6

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