Hypophysiotropic thyrotropin-releasing hormone-synthesizing neurons in the human hypothalamus are innervated by neuropeptide Y, agouti-related protein, and α-melanocyte-stimulating hormone

被引:94
作者
Mihály, E
Fekete, C
Tatro, JB
Liposits, Z
Stopa, EG
Lechan, RM
机构
[1] Tufts Univ New England Med Ctr, Tupper Res Inst, Boston, MA 02111 USA
[2] Tufts Univ New England Med Ctr, Div Endocrinol Diabet Metab & Mol Med, Dept Med, Boston, MA 02111 USA
[3] Hungarian Acad Sci, Inst Expt Med, Dept Neurobiol, H-1083 Budapest, Hungary
[4] Brown Univ, Rhode Isl Hosp, Sch Med, Dept Pathol,Neuropathol Div, Providence, RI 02903 USA
[5] Tufts Univ, Sch Med, Dept Neurosci, Boston, MA 02111 USA
关键词
D O I
10.1210/jc.85.7.2596
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We recently demonstrated that three arcuate nucleus-derived peptides, neuropeptide Y (NPY), agouti-related protein (AGRP), and alpha MSH, are contained in axon terminals that heavily innervate hypophysiotropic TRH neurons in the rat brain and may contribute to the altered set-point of the hypothalamo-pituitary-thyroid axis during fasting. To determine whether a similar regulatory system exists in human brain, we performed a series of immunohistochemical studies using antisera against NPY, AGRP, alpha MSH, and TRH in adult hypothalami obtained within 15 h of death. Numerous small to medium-sized, fusiform and multipolar NPY-, AGRP-, and alpha MSH-immunoreactive (-IR) cells were widely distributed throughout the rostro-caudal extent of the infundibular (arcuate) nucleus. A similar distribution pattern was found for NPY- and AGRP-IR neurons in the arcuate nucleus, whereas alpha MSH-IR cells appeared to form a separate cell population. By double labeling fluorescent immunohistochemistry, 82% of NPY neurons cocontained AGRP, and 87% of AGRP neurons coexpressed NPY. No colocalization was found between alpha MSH-and AGRP-IR neurons. NPY-, AGRP-, and alpha MSH-containing axons densely innervated the hypothalamic paraventricular nucleus and were found in close juxtaposition to TRH-synthesizing cell bodies and dendrites. These studies demonstrate that in man, the NPY-, AGRP-, and alpha MSK-IR neuronal systems in the infundibular and paraventricular nuclei are highly reminiscent of that observed in the rat and may similarly be involved in regulating the hypothalamo-pituitary-thyroid axis in the human brain.
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页码:2596 / 2603
页数:8
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