3′-processing of yeast tRNATrp precedes 5′-processing

被引：36

作者：

Kufel, J ^{[1
]}

Tollervey, D ^{[1
]}

机构：

[1] Univ Edinburgh, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland

来源：

RNA | 2003年 / 9卷 / 02期

关键词：

tRNA processing; La protein; Lsm proteins; Saccharomyces cerevisiae;

D O I：

10.1261/rna.2145103

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous analyses of eukaryotic pre-tRNAs processing have reported that 5'-cleavage by RNase P precedes 3'-maturation. Here we report that in contrast to all other yeast tRNAs analyzed to date, tRNA(Trp) undergoes 3'-maturation prior to 5'-cleavage. Despite its unusual processing pathway, pre-tRNA(TrP) resembles other pre-tRNAs, showing dependence on the essential Lsm proteins for normal processing and efficient association with the yeast La homolog, Lhp1p. tRNA T,p is also unusual in not requiring Lhp1p for 3' processing and stability. However, other Lhp1p-independent tRNAs, tRNA(2)(Lys) and tRNA(1)(Ile) follow the normal pathway of 5'-processing prior to 3-processing.

引用

页码：202 / 208

页数：7

共 48 条

[1] A doughnut-shaped heteromer of human Sm-like proteins binds to the 3′-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro [J].

Achsel, T ;

Brahms, H ;

Kastner, B ;

Bachi, A ;

Wilm, M ;

Lührmann, R .

EMBO JOURNAL, 1999, 18 (20) :5789-5802

[2] The Sm domain is an ancient RNA-binding motif with oligo(U) specificity [J].

Achsel, T ;

Stark, H ;

Lührmann, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (07) :3685-3689

[3]

Altman Sidney, 1995, P67

[4] Partial purification and characterization of nuclear ribonuclease P from wheat [J].

Arends, S ;

Schon, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 244 (02) :635-645

[5] IDENTIFICATION AND FUNCTIONAL-ANALYSIS OF 2 U3 BINDING-SITES ON YEAST PRERIBOSOMAL RNA [J].

BELTRAME, M ;

TOLLERVEY, D .

EMBO JOURNAL, 1992, 11 (04) :1531-1542

[6]

CASTANO JG, 1985, J BIOL CHEM, V260, P9002

[7]

CHEN JY, 1988, J BIOL CHEM, V263, P13677

[8] Crystal structure of a heptameric Sm-like protein complex from archaea: Implications for the structure and evolution of snRNPs [J].

Collins, BM ;

Harrop, SJ ;

Kornfeld, GD ;

Dawes, IW ;

Curmi, PMG ;

Mabbutt, BC .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 309 (04) :915-923

[9] IDENTIFICATION AND CHARACTERIZATION OF USS1P (SDB23P) - A NOVEL U6 SNRNA-ASSOCIATED PROTEIN WITH SIGNIFICANT SIMILARITY TO CORE PROTEINS OF SMALL NUCLEAR RIBONUCLEOPROTEINS [J].

COOPER, M ;

JOHNSTON, LH ;

BEGGS, JD .

EMBO JOURNAL, 1995, 14 (09) :2066-2075

[10]

ENGELKE DR, 1985, J BIOL CHEM, V260, P1271

← 1 2 3 4 5 →