Comparing the effects of resource enrichment and grazing on a bacterioplankton community of a meso-eutrophic reservoir

被引:70
作者
Simek, K
Hornák, K
Masín, M
Christaki, U
Nedoma, J
Weinbauer, MG
Dolan, JR
机构
[1] Acad Sci Czech Republ, Inst Hydrobiol, Ceske Budejovice, Czech Republic
[2] Univ S Bohemia, Fac Biol Sci, Ceske Budejovice, Czech Republic
[3] Natl Ctr Marine Res, Inst Oceanog, Athens 19013, Greece
[4] Netherlands Inst Sea Res, Dept Biol Oceanog, NL-1790 AB Den Burg, Netherlands
[5] UPMC, UMR CNRS 7093, Lab Oceanog Villefranche, Marine Microbial Ecol Grp, F-06234 Villefranche Sur Mer, France
关键词
reservoir; top-down and bottom-up control; dialysis bags; microbial food webs; bacterivory; bacterial community composition; RNA-targeted oligonucleotide probes;
D O I
10.3354/ame031123
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
We examined changes in a microbial community in reaction to shifts in predation pressure and resources in a meso-eutrophic reservoir. Manipulations consisted of size fractionation and either in situ incubation or transplantation from the dam area to the river area, which differed in P-availability. Three treatments, in which samples were incubated for 96 h in dialysis bags, were used: a bacterivore-free (<0.8 mu m) treatment, an enhanced flagellate grazing (<5 mum) treatment and a control treatment (all bacterivores present). We monitored bacterial abundance, mean cell volume (MCV), bacterial production, protistan grazing, as well as bacterial community composition (BCC) using fluorescence in situ hybridisation (FISH) with oligonucleotide probes with different levels of specificity. Populations transplanted to the relatively P-rich river area showed a 3- to 6-fold increases in bacterial and heterotrophic nanoflagellates (HNF) abundance and bacterivory compared to the corresponding treatments incubated at the dam area. In the transplanted community, nearly all phylogenetic groups of bacteria distinguished showed increased growth rates, even in the top-down manipulation treatments of increased bacterivory. in contrast, at the more resource-limited dam, the top-down manipulations induced significant changes in bacterial community composition. Thus, we found that BCC changes with predation in resource-limited bacteria but that predation plays a minor role when resource limitation is relaxed.
引用
收藏
页码:123 / 135
页数:13
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