Separation of long-range human TERT gene haplotypes by transformation-associated recombination cloning in yeast

被引：14

作者：

Kim, JH

Leem, SH

Sunwoo, Y

Kouprina, N

机构：

[1] NCI, Lab Biosyst & Canc, NIH, Bethesda, MD 20892 USA

[2] Dong A Univ, Dept Biol, Pusan 604714, South Korea

来源：

ONCOGENE | 2003年 / 22卷 / 16期

关键词：

hTERT; VNTR; polymorphism; haplotypes; TAR cloning;

D O I：

10.1038/sj.onc.1206316

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The hTERT gene encoding a catalytic subunit of human telomerase contains four blocks of variable number of tandem repeats (VNTRs) - two in intron 2 and two in intron 6. The segregation of hTERT VNTRs was analysed in families, revealing that all of them were transmitted through meiosis following a Mendelian inheritance. The work reports a further characterization of the minisatellites in hTERT. We employed transformation-associated recombination (TAR) cloning to isolate parental hTERT alleles and determined the specific combination of minisatellites at each of the polymorphic sites. A long-range haplotyping of hTERT determined by TAR cloning was verified by classical Mendelian analysis. Since such a strategy can be applied for any chromosomal locus, we conclude that recombinational gene capture could greatly facilitate haplotypes analysis.

引用

页码：2452 / 2456

页数：5

共 22 条

[1]

Chiu CP, 1997, P SOC EXP BIOL MED, V214, P99

[2]

FUKE S, 2001, PHARMACOGENOMICS J, V2, P152

[3]

REPEAT UNIT SEQUENCE VARIATION IN MINISATELLITES - A NOVEL SOURCE OF DNA POLYMORPHISM FOR STUDYING VARIATION AND MUTATION BY SINGLE MOLECULE ANALYSIS [J].