Interaction with avian cells and colonisation of specific pathogen free chicks by Shiga-toxin negative Escherichia coli O157:H7 (NCTC 12900)

被引:21
作者
Best, A
La Ragione, RM
Cooley, WA
O'Connor, CD
Velge, P
Woodward, MJ
机构
[1] Vet Labs Agcy, Dept Bacterial Dis, Addlestone KT15 3NB, Surrey, England
[2] Univ Southampton, Sch Biol Sci, Dept Mol Biol & Biochem, Southampton SO16 7BX, Hants, England
[3] Vet Labs Agcy, TMB Dept, Addlestone KT15 3NB, Surrey, England
[4] INRA Tours, Nouzilly Lab, F-37380 Nouzilly, France
基金
英国生物技术与生命科学研究理事会;
关键词
Escherichia coli O157 : H7; chicken; persistent infection; avian cell line; adherence;
D O I
10.1016/S0378-1135(03)00031-2
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The prevalence of Escherichia coli O157:H7 infection in birds is low but several deliberate inoculation studies show that poultry are readily and persistently infected by this organism indicating a possible threat to public health. The mechanisms of colonisation of poultry are not understood and the aim is to establish models to study the interaction of E. coli O157:H7, at the cellular and whole animal levels. A non-toxigenic E. coli O157:H7 (NCTC 12900) was used in adherence assays with an avian epithelial cell line (Div-1) and used to inoculate 1-day-old SPF chicks. In vitro, NCTC 12900 induced micro-colonies associated with cytoskeletal arrangements and pedestal formation with intimate bacterial attachment. In the 1-day-old SPF chick, a dose of 1 x 10(5) cfu resulted in rapid and extensive colonisation of the gastrointestinal tract and transient colonisation of the liver and spleen. The number of E. coli O157:H7 organisms attained approximately 10(8) cfu/ml caecal homogenate 24 h after inoculation and approximately 10(7) cfu/ml caecal homogenate was still present at day 92. Faecal shedding persisted for 169 days, ceasing 9 days after the birds came into lay and 6% of eggs were contaminated on the eggshell. Histological analysis of tissue samples from birds dosed with 1 x 10(7) cfu gave evidence for E coli O157:H7 NCTC 12900 induced micro-colonies on the caecal mucosa, although evidence for attaching effacing lesions was equivocal. These models may be suitable to study those factors of E. coli O157:H7 that mediate persistent colonisation in avian species. Crown Copyright (C) 2003 Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:207 / 222
页数:16
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