Effects of combined BDNF and GDNF treatment on cultured dopaminergic midbrain neurons

被引:37
作者
Sautter, J
Meyer, M
Spenger, C
Seiler, RW
Widmer, HR [1 ]
机构
[1] Univ Bern, Inselspital, Dept Neurosurg, CH-3010 Bern, Switzerland
[2] Odense Univ, Dept Anat & Cell Biol, Odense, Denmark
关键词
dopamine; graft; neurotrophic factor; Parkinson's disease; rat; tissue culture; transplantation; ventral mesencephalon;
D O I
10.1097/00001756-199804200-00025
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
NEURAL transplantation is an experimental therapy for Parkinson's disease. Pretreatment of fetal donor tissue with neurotrophic factors may improve survival of grafted dopaminergic neurons. Free-floating roller tube cultures of fetal rat ventral mesencephalon were treated with brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), or a combination of both. Dopamine content of the culture medium, the number of tyrosine hydroxylase-immunoreactive neurons, and culture volumes were moderately increased in the BDNF- and GDNF-treated cultures but significantly increased by 6.8-, 3.2- and 2.4-fold, respectively after treatment with the combination of both factors. We conclude that pretreatment of dopaminergic tissue in culture with a combination of BDNF and GDNF may be an effective means to improve the quality of tissue prior to grafting. (C) 1998 Rapid Science Ltd.
引用
收藏
页码:1093 / 1096
页数:4
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