Plasma agents in bio-decontamination by dc discharges in atmospheric air

被引:113
作者
Machala, Zdenko [1 ]
Chladekova, Lenka [1 ]
Pelach, Michal [1 ]
机构
[1] Comenius Univ, Fac Math Phys & Informat, Div Environm Phys, Bratislava 84248, Slovakia
关键词
DIELECTRIC BARRIER DISCHARGES; PRESSURE PLASMA; GLOW-DISCHARGE; NONTHERMAL PLASMA; ESCHERICHIA-COLI; STERILIZATION; INACTIVATION; BACTERIA; NEEDLE; DEACTIVATION;
D O I
10.1088/0022-3727/43/22/222001
中图分类号
O59 [应用物理学];
学科分类号
摘要
Bio-decontamination of water and surfaces contaminated by bacteria (Salmonella typhimurium) was investigated in two types of positive dc discharges in atmospheric pressure air, in needle-to-plane geometry: the streamer corona and its transition to a novel regime called transient spark with short high current pulses of limited energy. Both generate a cold non-equilibrium plasma. Electro-spraying of treated water through a needle electrode was applied for the first time and resulted in fast bio-decontamination. Experiments providing separation of various biocidal plasma agents, along with the emission spectra and coupled with oxidation stress measurements in the cell membranes helped to better understand the mechanisms of microbial inactivation. The indirect exposure of contaminated surfaces to neutral active species was almost as efficient as the direct exposure to the plasma, whereas applying only UV radiation from the plasma had no biocidal effects. Radicals and reactive oxygen species were identified as dominant biocidal agents.
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页数:7
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