Recent advances in DNA sequencing methods - general principles of sample preparation

被引：50

作者：

Linnarsson, Sten ^{[1
]}

机构：

[1] Karolinska Inst, Mol Neurobiol Lab, Dept Med Biochem & Biophys, SE-17177 Stockholm, Sweden

来源：

EXPERIMENTAL CELL RESEARCH | 2010年 / 316卷 / 08期

关键词：

DNA sequencing; Sample preparation; RNA-Seq; SINGLE-CELL GENOMICS; AMPLIFICATION-FREE; RNA-SEQ;

D O I：

10.1016/j.yexcr.2010.02.036

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

DNA sequencing has revolutionized biomedicine, and progress in the field has been unrelenting since it was invented over 30 years ago. The complete DNA sequence of the human genome was obtained as the culmination of a decade of work by a large number of scientists. Less than ten years later, so-called 'next-generation' instruments now make it possible for a single lab to produce the same amount of data in a week. But while the instruments are increasingly automated, upstream sample processing remains a challenge. Here I review the current state of the art in preparing genomic and RNA samples for high throughput sequencing. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：1339 / 1343

页数：5

共 31 条

[1]

BING DH, 1996, GEN ID C P 7 INT S H

[2] Stem cell transcriptome profiling via massive-scale mRNA sequencing [J].

Cloonan, Nicole ;

Forrest, Alistair R. R. ;

Kolle, Gabriel ;

Gardiner, Brooke B. A. ;

Faulkner, Geoffrey J. ;

Brown, Mellissa K. ;

Taylor, Darrin F. ;

Steptoe, Anita L. ;

Wani, Shivangi ;

Bethel, Graeme ;

Robertson, Alan J. ;

Perkins, Andrew C. ;

Bruce, Stephen J. ;

Lee, Clarence C. ;

Ranade, Swati S. ;

Peckham, Heather E. ;

Manning, Jonathan M. ;

McKernan, Kevin J. ;

Grimmond, Sean M. .

NATURE METHODS, 2008, 5 (07) :613-619

[3] Nascent RNA Sequencing Reveals Widespread Pausing and Divergent Initiation at Human Promoters [J].

Core, Leighton J. ;

Waterfall, Joshua J. ;

Lis, John T. .

SCIENCE, 2008, 322 (5909) :1845-1848

[4] A simple method for directional transcriptome sequencing using Illumina technology [J].

Croucher, Nicholas J. ;

Fookes, Maria C. ;

Perkins, Timothy T. ;

Turner, Daniel J. ;

Marguerat, Samuel B. ;

Keane, Thomas ;

Quail, Michael A. ;

He, Miao ;

Assefa, Sammey ;

Baehler, Juerg ;

Kingsley, Robert A. ;

Parkhill, Julian ;

Bentley, Stephen D. ;

Dougan, Gordon ;

Thomson, Nicholas R. .

NUCLEIC ACIDS RESEARCH, 2009, 37 (22) :e148-e148

[5] The challenges of sequencing by synthesis [J].

Fuller, Carl W. ;

Middendorf, Lyle R. ;

Benner, Steven A. ;

Church, George M. ;

Harris, Timothy ;

Huang, Xiaohua ;

Jovanovich, Stevan B. ;

Nelson, John R. ;

Schloss, Jeffery A. ;

Schwartz, David C. ;

Vezenov, Dmitri V. .

NATURE BIOTECHNOLOGY, 2009, 27 (11) :1013-1023

[6]

GAILLARD C, 1998, ELSEVIER TRENDS J, P1

[7] Directed evolution of polymerase function by compartmentalized self-replication [J].

Ghadessy, FJ ;

Ong, JL ;

Holliger, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (08) :4552-4557

[8] Single-cell genomics [J].

Hutchison, Clyde A., III ;

Venter, J. Craig .

NATURE BIOTECHNOLOGY, 2006, 24 (06) :657-658

[9]

Kozarewa I, 2009, NAT METHODS, V6, P291, DOI [10.1038/NMETH.1311, 10.1038/nmeth.1311]

[10] Highly integrated single-base resolution maps of the epigenome in Arabidopsis [J].

Lister, Ryan ;

O'Malley, Ronan C. ;

Tonti-Filippini, Julian ;

Gregory, Brian D. ;

Berry, Charles C. ;

Millar, A. Harvey ;

Ecker, Joseph R. .

CELL, 2008, 133 (03) :523-536

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