Highly sensitive electrochemical determination of Escherichia coli density using tyrosinase-based chemically amplified biosensor

被引：27

作者：

Hasebe, Y ^{[1
]}

Yokobori, K ^{[1
]}

Fukasawa, K ^{[1
]}

Kogure, T ^{[1
]}

Uchiyama, S ^{[1
]}

机构：

[1] Saitama Inst Technol, Dept Environm Engn, Okabe, Saitama 36902, Japan

来源：

ANALYTICA CHIMICA ACTA | 1997年 / 357卷 / 1-2期

关键词：

Escherichia coli density; tyrosinase; substrate recycling; L-ascorbic acid; biosensor;

D O I：

10.1016/S0003-2670(97)00544-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Tyrosinase-based chemically amplified biosensors, based on a substrate recycling of polyphenolic compounds driven by tyrosinase-catalyzed oxidation and chemical reduction of o-quinones by L-ascorbic acid (AsA), has been applied for the highly sensitive electrochemical determination of the Escherichia coli (E. coli) density. The amplified signal of the tyrosinase-coupling electrode due to the recycling of tyrosinase-active polyphenolic compounds, which is microbially generated from the salicylic acid derivatives by the metabolic pathway of E. coli, depends considerably on the E. coli density ranging from 10(3) to 10(4) cells/ml level. (C) 1997 Elsevier Science B.V.

引用

页码：51 / 54

页数：4

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