Kinetic modeling using S-systems and lin-log approaches

We analyze the growth dynamics and production of an industrially interesting amino acid in a recombinant Escherichia coli strain, using in parallel two alternative modeling frameworks, namely S-systems and lin-log models. These models have identical steady-state solutions, but differ in their representation of transients. The models were parameterized with data from two bioprocesses that differed in their initial culture concentrations and then used to predict responses under yet a different initial culture regimen. Among the S-system models, several alternatives representing slightly different pathway structures, were tested. All were found to be capable of capturing the dynamics of all variables in the test systems and also of predicting the dynamic responses under new conditions. The lin-log models also captured the dynamics, but not as well as the S-system models. A probable reason for the inferior performance of lin-log models is their intrinsic property of not representing situations well where variable concentrations are moderately small. (c) 2006 Elsevier B.V. All rights reserved.