Tubulin is hyperphosphorylated on serine and tyrosine residues in arsenite-resistant Leishmania donovani promastigotes

被引：15

作者：

Prasad, V ^{[1
]}

Dey, CS ^{[1
]}

机构：

[1] Natl Inst Pharmaceut Educ & Res, Dept Biotechnol, SAS Nagar 160062, Punjab, India

来源：

PARASITOLOGY RESEARCH | 2000年 / 86卷 / 11期

关键词：

D O I：

10.1007/s004360000249

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

An arsenite-resistant strain of Leishmania donovani was generated in vitro by the sequential exposure of a wild type strain to increasing concentrations of sodium m-arsenite. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of whole cell lysates of the two strains revealed that a protein band at the 55 kDa position showed slower migration in the resistant samples. This band was identified as tubulin by immunoblotting, with both alpha- and beta -tubulin showing retarded migration in the resistant strain. Investigations into the reason for the observed slower migration revealed that phosphorylation of tubulin on both serine and tyrosine residues was enhanced in the resistant strain when compared to the wild type strain.

引用

页码：876 / 880

页数：5

共 33 条

[1] PARTIAL-PURIFICATION AND CHARACTERIZATION OF A SOLUBLE-PROTEIN KINASE FROM LEISHMANIA-DONOVANI PROMASTIGOTES [J].

BANERJEE, C ;

SARKAR, D .

JOURNAL OF GENERAL MICROBIOLOGY, 1990, 136 :1051-1057

[2] Chemosensitization of cancer cells by the staurosporine derivative CGP 41251 in association with decreased P-glycoprotein phosphorylation [J].

Beltran, PJ ;

Fan, D ;

Fidler, IJ ;

OBrian, CA .

BIOCHEMICAL PHARMACOLOGY, 1997, 53 (02) :245-247

[3] INHIBITION OF LEISHMANIAL PROTEIN-KINASE BY ANTILEISHMANIAL DRUGS [J].

BERMAN, JD .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1988, 38 (02) :298-303

[4]

BLOBE GC, 1993, J BIOL CHEM, V268, P658

[5] Binding and phosphorylation of tubulin by G protein-coupled receptor kinases [J].

Carman, CV ;

Som, T ;

Kim, CM ;

Benovic, JL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (32) :20308-20316

[6]

CHAMBERS TC, 1990, J BIOL CHEM, V265, P7679

[7] INHIBITION OF LEISHMANIAS BUT NOT HOST MACROPHAGES BY THE ANTITUBULIN HERBICIDE TRIFLURALIN [J].

CHAN, MMY ;

FONG, D .

SCIENCE, 1990, 249 (4971) :924-926

[8] SIGNALING AND DRUG-SENSITIVITY [J].

CHRISTEN, RD ;

ISONISHI, S ;

JONES, JA ;

JEKUNEN, AP ;

HOM, DK ;

KRONING, R ;

GATELY, DP ;

THIEBAUT, FB ;

LOS, G ;

HOWELL, SB .

CANCER AND METASTASIS REVIEWS, 1994, 13 (02) :175-189

[9] A CYCLIC NUCLEOTIDE-INDEPENDENT PROTEIN-KINASE IN LEISHMANIA-DONOVANI [J].

DAS, S ;

SAHA, AK ;

MUKHOPADHYAY, NK ;

GLEW, RH .

BIOCHEMICAL JOURNAL, 1986, 240 (03) :641-649

[10] STAGE-SPECIFIC REGULATION OF PROTEIN-PHOSPHORYLATION IN LEISHMANIA-MAJOR [J].

DELL, KR ;

ENGEL, JN .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1994, 64 (02) :283-292

← 1 2 3 4 →