Ultrasound biomicroscopy permits in vivo characterization of zebrafish liver tumors

被引：76

作者：

Goessling, Wolfram

North, Trista E.

Zon, Leonard I. ^{[1
]}

机构：

[1] Childrens Hosp, Stem Cell Program, Boston, MA 02115 USA

[2] Harvard Univ, Dana Farber Canc Inst, Howard Hughes Med Inst, Stem Cell Inst,Med Sch, Boston, MA 02115 USA

[3] Massachusetts Gen Hosp, Gastrointestinal Unit, Boston, MA 02114 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 07期

关键词：

THERAPY;

D O I：

10.1038/NMETH1059

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Zebrafish are a valuable vertebrate model to study carcinogenesis, but noninvasive imaging is challenging because adult fish are not transparent. Here we show that tumors could be readily detected in vivo using high-resolution microscopic ultrasound in zebrafish. We successfully obtained tissue perfusion calculations and cellular aspirates, and analyzed tumor progression and response to treatment. Ultrasound biomicroscopy allows longitudinal studies of tumor development and real-time assessment of therapeutic effects in zebrafish.

引用

收藏

页码：551 / 553

页数：3

相关论文

共 12 条

[1] tp53 mutant zebrafish develop malignant peripheral nerve sheath tumors [J].

Berghmans, S ;

Murphey, RD ;

Wienholds, E ;

Neuberg, D ;

Kutok, JL ;

Fletcher, CDM ;

Morris, JP ;

Liu, TX ;

Schulte-Merker, S ;

Kanki, JP ;

Plasterk, R ;

Zon, LI ;

Look, AT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (02) :407-412

[2] Advances in ultrasound biomicroscopy [J].

Foster, FS ;

Pavlin, CJ ;

Harasiewicz, KA ;

Christopher, DA ;

Turnbull, DH .

ULTRASOUND IN MEDICINE AND BIOLOGY, 2000, 26 (01) :1-27

[3] Targeted anti-vascular endothelial growth factor receptor-2 therapy leads to short-term and long-term impairment of vascular function and increase in tumor hypoxia [J].

Franco, M ;

Man, S ;

Chen, L ;

Emmenegger, U ;

Shaked, Y ;

Cheung, AM ;

Brown, AS ;

Hicklin, DJ ;

Foster, FS ;

Kerbel, RS .

CANCER RESEARCH, 2006, 66 (07) :3639-3648

[4] High frequency nonlinear scattering from a micrometer to submicrometer sized lipid encapsulated contrast agent [J].

Goertz, DE ;

Frijlink, ME ;

De Jong, N ;

Steen, AFWV .

ULTRASOUND IN MEDICINE AND BIOLOGY, 2006, 32 (04) :569-577

[5] High-frequency 3-D color-flow imaging of the microcirculation [J].

Goertz, DE ;

Yu, JL ;

Kerbel, RS ;

Burns, PN ;

Foster, FS .

ULTRASOUND IN MEDICINE AND BIOLOGY, 2003, 29 (01) :39-51

[6]

Goertz DE, 2002, CANCER RES, V62, P6371

[7]

GOESSLING W, IN PRESS J CLIN ONCO

[8] High-frequency ultrasound detection and follow-up of Wilms' tumor in the mouse [J].

Jouannot, E ;

Duong-Van-Huyen, JP ;

Bourahla, K ;

Laugier, P ;

Lelievre-Pegorier, M ;

Bridal, L .

ULTRASOUND IN MEDICINE AND BIOLOGY, 2006, 32 (02) :183-190

[9] Conservation of gene expression signatures between zebrafish and human liver tumors and tumor progression [J].

Lam, SH ;

Wu, YL ;

Vega, VB ;

Miller, LD ;

Spitsbergen, J ;

Tong, Y ;

Zhan, HQ ;

Govindarajan, KR ;

Lee, S ;

Mathavan, S ;

Murthy, KRK ;

Buhler, DR ;

Liu, ET ;

Gong, ZY .

NATURE BIOTECHNOLOGY, 2006, 24 (01) :73-75

[10] Microbubbles in medical imaging: current applications and future directions [J].

Lindner, JR .

NATURE REVIEWS DRUG DISCOVERY, 2004, 3 (06) :527-532