Hepatocyte growth factor receptor is a coreceptor for adeno-associated virus type 2 infection

被引：173

作者：

Kashiwakura, Y

Tamayose, K

Iwabuchi, K

Hirai, Y

Shimada, T

Matsumoto, K

Nakamura, T

Watanabe, M

Oshimi, K

Daida, H

机构：

[1] Juntendo Univ, Sch Med, Dept Hematol, Bunkyo Ku, Tokyo 1138421, Japan

[2] Juntendo Univ, Sch Med, Dept Cardiol, Tokyo, Japan

[3] Nippon Med Coll, Dept Biochem & Mol Biol, Tokyo, Japan

[4] Juntendo Univ, Grad Sch Med, Inst Environm & Gender Specif Med, Chiba, Japan

[5] Osaka Univ, Grad Sch Med, Div Mol Regenerat Med, Course Adv Med, Osaka, Japan

[6] Okayama Univ, Grad Sch Med & Dent, Dept Urol, Okayama, Japan

来源：

JOURNAL OF VIROLOGY | 2005年 / 79卷 / 01期

关键词：

D O I：

10.1128/JVI.79.1.609-614.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

After the first attachment of virus to the cell surface through a primary receptor, efficient entry of virus requires the presence of a coreceptor. For adeno-associated virus type 2 (AAV2) infection, heparan sulfate proteoglycan is supposed as the primary receptor, and alphavbeta5 integrin and FGFR1 are reported to act as coreceptors. In this study, we were able to demonstrate that hepatocyte growth factor receptor, e-Met, is also a coreceptor for AAV2 infection. AAV2-mediated transgene analyses revealed that c-Met expression significantly up-regulated transgene expression without increasing AAV2 cell binding. Moreover, a viral overlay assay elucidated the physical interaction between AAV2 and the beta subunit of c-Met. These data suggest that c-Met plays the role of coreceptor for AAV2 infection by facilitating AAV2 internalization into the cytoplasm.

引用

页码：609 / 614

页数：6

共 35 条

[1] Mitotic activity of rat muscle satellite cells in response to serum stimulation: relation with cellular metabolism [J].