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TaqMan PCR for detection of genetically modified durum wheat

被引：16
作者：
Terzi, V
Ferrari, B
Finocchiaro, F
Di Fonzo, N
Stanca, AM
Lamacchia, C
Napier, J
Shewry, PR
Faccioli, P
机构：
[1] Ist Sperimentale Cerealicoltura, I-29017 Fiorenzuola Darda, PC, Italy
[2] Ist Sperimentale Cerealicolultra, I-61100 Foggia, Italy
[3] Univ Bristol, Long Ashton Res Stn, IACR, Dept Agr Sci, Bristol BS18 9AF, Avon, England
来源：
JOURNAL OF CEREAL SCIENCE | 2003年 / 37卷 / 02期
关键词：
durum wheat; GMO detection; real time PCR;
D O I：
10.1006/jcrs.2002.0489
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
In recent years food crops have been engineered to express novel genes which impart new characteristics. However, according to EU regulations, novel foods and ingredients must be labelled to allow consumers to make informed decisions regarding the foodstuffs they purchase. Analytical methods are thus necessary to show the presence or absence of GMO in raw materials and food. New transformation events are routinely submitted to the. EU Council and analytical methods therefore need to be continuously up-dated. Consequently, the aim of this work has been to develop a qualitative and quantitative PCR method to detect a genetically modified durum wheat. The use of the 5700 Sequence Detection System (PE Biosystems) allowed the determination of the amplified product accumulation using a fluorogenic probe (TaqMan). (C) 2002 Elsevier Science Ltd. All rights reserved.
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收藏
页码：157 / 163
页数：7
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