Development of diagnostic PCR markers closely linked to the tomato powdery mildew resistance gene Ol-1 on chromosome 6 of tomato

被引:31
作者
Huang, CC
Cui, YY
Weng, CR
Zabel, P
Lindhout, P
机构
[1] Univ Wageningen & Res Ctr, Grad Sch Expt Plant Sci, Lab Plant Breeding, NL-6700 AJ Wageningen, Netherlands
[2] Univ Wageningen & Res Ctr, Grad Sch Expt Plant Sci, Mol Biol Lab, NL-6700 AJ Wageningen, Netherlands
[3] Univ Missouri, Coll Agr Food & Nat Resources, Dept Plant Pathol, Plant Sci Unit, Columbia, MO 65211 USA
关键词
resistance; tomato powdery mildew; tomato; mapping; Oidium lycopersicum; RFLP; sequence characterised amplified region (SCAR);
D O I
10.1007/s001220051562
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Lycopersicon hirsutum G1.1560 is a wild accession of tomato that shows resistance to Oidium lycopersicum, a frequently occurring tomato powdery mildew. This resistance is largely controlled by an incompletely dominant gene Ol-1 near the Aps-l locus in the vicinity of the resistance,genes Mi and Cf-2/Cf-5. Using a new F-2 population (n=150) segregating for resistance, we mapped the Ol-1 gene more accurately to a location between the RFLP markers TG153 and TG164. Furthermore, in saturating the Ol-1 region with more molecular markers using bulked segregant analysis, we were able to identify five RAPDs associated with the resistance. These RAPDs were then sequenced and converted into SCAR markers: SCAB01 and SCAF10 were L. hirsutum-specific; SCAE16, SCAG11 and SCAK16 were L. esculentum-specific. By linkage analysis a dense integrated map comprising RFLP and SCAR markers near Ol-1 was obtained. This will facilitate a map-based cloning approach for Ol-1 and marker-assisted selection for powdery mildew resistance in tomato breeding.
引用
收藏
页码:918 / 924
页数:7
相关论文
共 40 条
[1]   ACID PHOSPHATASE-11, A TIGHTLY LINKED MOLECULAR MARKER FOR ROOT-KNOT NEMATODE RESISTANCE IN TOMATO - FROM PROTEIN TO GENE, USING PCR AND DEGENERATE PRIMERS CONTAINING DEOXYINOSINE [J].
AARTS, JMMJG ;
HONTELEZ, JGJ ;
FISCHER, P ;
VERKERK, R ;
VANKAMMEN, A ;
ZABEL, P .
PLANT MOLECULAR BIOLOGY, 1991, 16 (04) :647-661
[2]  
BEDENES C, 1996, SOMATIC CELL GENETIC, P239
[3]   Use of a SCAR-based marker for the early detection of dwarf off-types in micropropagated Cavendish bananas [J].
Damasco, OP ;
Adkins, SW ;
Godwin, ID ;
Smith, MK .
INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY OF TROPICAL AND SUBTROPICAL SPECIES - PART II, 1998, (461) :157-164
[4]   Development and characterization of SCAB markers linked to the citrus tristeza virus resistance gene from Poncirus trifoliata [J].
Deng, ZN ;
Huang, S ;
Xiao, SY ;
Gmitter, FG .
GENOME, 1997, 40 (05) :697-704
[5]   The tomato Cf-5 disease resistance gene and six homologs show pronounced allelic variation in leucine-rich repeat copy number [J].
Dixon, MS ;
Hatzixanthis, K ;
Jones, DA ;
Harrison, K ;
Jones, JDG .
PLANT CELL, 1998, 10 (11) :1915-1925
[6]   The tomato Cf-2 disease resistance locus comprises two functional genes encoding leucine-rich repeat proteins [J].
Dixon, MS ;
Jones, DA ;
Keddie, JS ;
Thomas, CM ;
Harrison, K ;
Jones, JDG .
CELL, 1996, 84 (03) :451-459
[7]   Development of molecular markers linked to a gene controlling fruit acidity in citrus [J].
Fang, DQ ;
Federici, CT ;
Roose, ML .
GENOME, 1997, 40 (06) :841-849
[8]   GENETIC-VARIATION IN HOMOTHALLIC AND HYPHAL SWELLING ISOLATES OF PYTHIUM-ULTIMUM VAR ULTIMUM AND P-ULTIMUM VAR SPORANGIFERUM [J].
FRANCIS, DM ;
GEHLEN, MF ;
STCLAIR, DA .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1994, 7 (06) :766-775
[9]   RFLP ANALYSIS AND LINKAGE MAPPING IN SOLANUM-TUBEROSUM [J].
GEBHARDT, C ;
RITTER, E ;
DEBENER, T ;
SCHACHTSCHABEL, U ;
WALKEMEIER, B ;
UHRIG, H ;
SALAMINI, F .
THEORETICAL AND APPLIED GENETICS, 1989, 78 (01) :65-75
[10]   A family of LRR sequences in the vicinity of the Co-2 locus for anthracnose resistance in Phaseolus vulgaris and its potential use in marker-assisted selection [J].
Geffroy, V ;
Creusot, F ;
Falquet, J ;
Sévignac, M ;
Adam-Blondon, AF ;
Bannerot, H ;
Gepts, P ;
Dron, M .
THEORETICAL AND APPLIED GENETICS, 1998, 96 (3-4) :494-502