Glucagon-like peptide-1 receptor expression in Xenopus oocytes stimulates inositol trisphosphate-dependent intracellular Ca2+ mobilization

被引：17

作者：

Gromada, J

Anker, C

Bokvist, K

Knudsen, LB

Wahl, P

机构：

[1] Novo Nordisk AS, Dept Islet Cell Physiol, DK-2100 Copenhagen, Denmark

[2] Novo Nordisk AS, Dept Mol Pharmacol, Copenhagen, Denmark

来源：

FEBS LETTERS | 1998年 / 425卷 / 02期

关键词：

glucagon-like peptide-1; calcium; inositol trisphosphate; Xenopus oocyte; cyclic adenosine monophosphate;

D O I：

10.1016/S0014-5793(98)00254-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The signal transduction pathway of the cloned human glucagon-like peptide-1 (GLP-1) receptor was studied in voltage-clamped Xenopus oocytes. Binding of GLP-1(7-36)amide was associated with cAMP production, increased [Ca2+](i) and activation of Ca2+-dependent Cl- current. The effect of GLP-1(7-36)amide reflects intracellular Ca2+ mobilization and was suppressed by injection of the Ca2+ chelator BAPTA and the inositol trisphosphate receptor antagonist heparin. The responses were not mimicked by the adenylate cyclase activator forskolin and unaffected by the protein kinase A (PKA) inhibitor Rp-cAMPS. We conclude that GLP-1 receptor expression in Xenopus oocytes evokes inositol trisphosphate-dependent intracellular Ca2+ mobilization independent of the cAMP/PKA signaling pathway. (C) 1998 Federation of European Biochemical Societies.

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页码：277 / 280

页数：4

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