Recombination dynamics of human parechoviruses: investigation of type-specific differences in frequency and epidemiological correlates

被引:59
作者
Calvert, J. [1 ]
Chieochansin, T. [1 ,2 ]
Benschop, K. S. [3 ]
Leitch, E. C. McWilliam [1 ]
Drexler, J. F. [4 ,5 ]
Grywna, K. [4 ]
Ribeiro, H. da Costa, Jr. [6 ]
Drosten, C. [4 ]
Harvala, H. [7 ]
Poovorawan, Y. [2 ]
Wolthers, K. C. [3 ]
Simmonds, P. [1 ]
机构
[1] Univ Edinburgh, Ctr Infect Dis, Edinburgh, Midlothian, Scotland
[2] Chulalongkorn Univ, Fac Med, Ctr Excellence Clin Virol, Bangkok 10330, Thailand
[3] Univ Amsterdam, Acad Med Ctr, Lab Clin Virol, NL-1105 AZ Amsterdam, Netherlands
[4] Inst Virol, Bonn, Germany
[5] Univ Fed Bahia, Infect Dis Res Lab, Univ Hosp Prof Edgard Santos, Salvador, BA, Brazil
[6] Univ Fed Bahia, Dept Paediat, Hosp Prof Edgar Santos, Salvador, BA, Brazil
[7] Royal Infirm Edinburgh NHS Trust, Virol Lab, Edinburgh, Midlothian, Scotland
关键词
MOLECULAR EPIDEMIOLOGY; EVOLUTION; IDENTIFICATION; ECHOVIRUS-22; ASSOCIATION; INFECTIONS; PREVALENCE; SELECTION; OUTBREAK; SEQUENCE;
D O I
10.1099/vir.0.018747-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Human parechoviruses (HPeVs) are highly prevalent RNA viruses classified in the family Picornaviridae. Several antigenically distinct types circulate in human populations worldwide, whilst recombination additionally contributes to the genetic heterogeneity of the virus. To investigate factors influencing the likelihood of recombination and to compare its dynamics among types, 154 variants collected from four widely geographically separated referral centres (UK, The Netherlands, Thailand and Brazil) were typed by VP3/VP1 amplification/sequencing with recombination groups assigned by analysis of 3Dpol sequences. HPeV1B and HPeV3 were the most frequently detected types in each referral region, but with marked geographical differences in the frequencies of different recombinant forms (RFs) of types 1B, 5 and 6. HPeV1B showed more frequent recombination than HPeV3, in terms both of evolutionary divergence and of temporal/geographical indicators of population separation. HPeV1 variants showing between 10 and 20% divergence in VP3/VP1 almost invariably fell into different recombination groups, compared with only one-third of similarly divergent HPeV3 variants. Substitution rates calculated by BEAST in the VP3/VP1 region of HPeV1 and HPeV3 allowed half-lives of the RFs of 4 and 20 years, respectively, to be calculated, estimates fitting closely with their observed lifespans based on population sampling. The variability in recombination dynamics between HPeV1B and HPeV3 offers an intriguing link with their markedly different seasonal patterns of transmission, age distributions of infection and clinical outcomes. Future investigation of the epidemiological and biological opportunities and constraints on intertypic recombination will provide more information about its influence on the longer term evolution and pathogenicity of parechoviruses.
引用
收藏
页码:1229 / 1238
页数:10
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