Effect of partial desulfation and oversulfation of sodium spirulan on the potency of anti-herpetic activities

被引：16

作者：

Lee, Jung-Bum ^{[1
]}

Hou, Xiaolon ^{[1
]}

Hayashi, Kyoko ^{[1
]}

Hayashi, Toshimitsu ^{[1
]}

机构：

[1] Toyama Univ, Grad Sch Med & Pharmaceut Sci, Toyama 9300194, Japan

来源：

CARBOHYDRATE POLYMERS | 2007年 / 69卷 / 04期

关键词：

sodium spirulan; Spirulina platensis; partial desulfation; oversulfation; antiviral activity;

D O I：

10.1016/j.carbpol.2007.01.024

中图分类号：

O69 [应用化学];

学科分类号：

081704 [应用化学];

摘要：

The aim of the present study is to evaluate the effect of partial desulfation and oversulfation of sodium spirulan (Na-SP) isolated from Spirulina platensis on the exhibition of anti-herpes simplex viruses type 1 and 2 (HSV-1 and -2) activities. Partially desulfated (PDS-SPs) and oversulfated derivatives (OS-SPs) were obtained by solvolytic desulfation and sulfation with dicyclocarbodiimide-sulfuric acid, respectively. When PDS-SPs were subjected to anti-HSV-1 assay, antiviral potency was dependent on their sulfate content, and PDSSPs with lower sulfate content than 8.6% were found to be inactive. Some derivatives showing anti-HSV-1 effect also showed anti-HSV-2 activity. Anti-HSV-1 effect of OS-SPs was equivalent to that of Na-SP when they were added to the medium during viral infection and throughout the incubation thereafter, while they were enhanced as compared with Na-SP when they were added to the medium immediately after viral infection. The results of time-of-addition experiments suggested that the most sensitive phase of OS-SP-2 and -5 might be the early steps of viral adsorption and penetration. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：651 / 658

页数：8

共 34 条

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