3D printed alendronate-releasing poly(caprolactone) porous scaffolds enhance osteogenic differentiation and bone formation in rat tibial defects

被引：45

作者：

Kim, Sung Eun ^{[1
,2
]}

Yun, Young-Pil ^{[1
,2
]}

Shim, Kyu-Sik ^{[3
]}

Kim, Hak-Jun ^{[1
,2
]}

Park, Kyeongsoon ^{[4
]}

Song, Hae-Ryong ^{[1
,2
]}

机构：

[1] Korea Univ, Med Coll, Guro Hosp, Dept Orthoped Surg, 80 Guro Dong, Seoul 152703, South Korea

[2] Korea Univ, Med Coll, Guro Hosp, Rare Dis Inst, 80 Guro Dong, Seoul 152703, South Korea

[3] Korea Univ, Coll Med, Dept Biomed Sci, Seoul 136701, South Korea

[4] Korea Basic Sci Inst, Div Bioimaging, Chuncheon Ctr, Gangwondaehakgil 1, Chunchon 200701, Gangwon Do, South Korea

来源：

BIOMEDICAL MATERIALS | 2016年 / 11卷 / 05期

关键词：

three-dimensional (3D) printed scaffold; alendronate; MG-63; cells; tibial defect model; bone formation; 3-DIMENSIONAL FIBER-DEPOSITION; STEM-CELLS; IN-VITRO; POLYCAPROLACTONE SCAFFOLDS; OSTEOBLAST PROLIFERATION; TISSUE REGENERATION; LOCAL-DELIVERY; DESIGN; STEREOLITHOGRAPHY; BISPHOSPHONATES;

D O I：

10.1088/1748-6041/11/5/055005

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

The aim of this study was to evaluate the in vitro osteogenic effects and in vivo new bone formation of three-dimensional (3D) printed alendronate (Aln)-releasing poly(caprolactone) (PCL) (Aln/PCL) scaffolds in rat tibial defect models. 3D printed Aln/PCL scaffolds were fabricated via layer-by-layer deposition. The fabricated Aln/PCL scaffolds had high porosity and an interconnected pore structure and showed sustained Aln release. In vitro studies showed that MG-63 cells seeded on the Aln/PCL scaffolds displayed increased alkaline phosphatase (ALP) activity and calcium content in a dose-dependent manner when compared with cell cultures in PCL scaffolds. In addition, in vivo animal studies and histologic evaluation showed that Aln/PCL scaffolds implanted in a rat tibial defect model markedly increased new bone formation and mineralized bone tissues in a dose-dependent manner compared to PCL-only scaffolds. Our results show that 3D printed Aln/PCL scaffolds are promising templates for bone tissue engineering applications.

引用

页数：11

共 60 条

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