Variations on the GFP chromophore

被引：70

作者：

Wilmann, PG

Petersen, J

Devenish, RJ

Prescott, M ^{[1
]}

Rossjohn, J

机构：

[1] Monash Univ, Sch Biomed Sci, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia

[2] Monash Univ, Monash Ctr Synchrotron Sci, Prot Crystallog Unit, Clayton, Vic 3800, Australia

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2005年 / 280卷 / 04期

关键词：

D O I：

10.1074/jbc.C400484200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have determined to 2.1 Angstrom resolution the crystal structure of a dark state, kindling fluorescent protein isolated from the sea anemone, Anemonia sulcata. The chromophore sequence Met(63)-Tyr(64)-Gly(65) of the A. sulcata chromoprotein was previously proposed to comprise a 6-membered pyrazine-type heterocycle (Martynov, V. I., Savitsky, A. P., Martynova, N. Y., Savitsky, P. A., Lukyanov, K. A., and Lukyanov, S. A. (2001) J. Biol. Chem. 2 76, 21012-21016). However, our crystallographic data revealed the chromophore to comprise a 5-membered p-hydroxybenzylideneimidazolinone moiety that adopts a non-coplanar trans conformation within the interior of the GFP beta-can fold. Unexpectedly, fragmentation of the polypeptide was found to occur within the chromophore moiety, at the bond between Cys(62C) and Met(63N1) Our structural data reveal that fragmentation of the chromophore represents an intrinsic, autocatalytic step toward the formation of the mature chromophore within the specific GFP-like proteins.

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页码：2401 / 2404

页数：4

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