Initial steps in the fermentation of 3-hydroxybenzoate by Sporotomaculum hydroxybenzoicum

被引:17
作者
Müller, JA [1 ]
Schink, B [1 ]
机构
[1] Univ Konstanz, Fak Biol, D-78457 Constance, Germany
关键词
anaerobic degradation; aromatic compounds; 3-hydroxybenzoate; benzoyl-CoA pathway; reductive dehydroxylation; fermenting bacteria; Sporotomaculum hydroxybenzoicum;
D O I
10.1007/s002030000148
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The anaerobic bacterium Sporotomaculum hydroxybenzoicum ferments 3-hydroxybenzoate to acetate, butyrate, and CO2. 3-Hydroxybenzoate was activated to 3-hydroxybenzoyl-CoA in a CoA-transferase reaction with acetyl-CoA or butyryl-CoA as CoA donors. 3-Hydroxybenzoyl-CoA was reductively dehydroxylated, forming benzoyl-CoA. This reaction was measured in cell-free extracts with cob(I)alamin as low-potential electron donor. No evidence was obtained that cob(I)alamin is the physiological electron donor; however, inhibitor studies indicated involvement of a strong nucleophile in the reaction. Benzoate was degraded by dense cell suspensions without a lag phase until an in situ DeltaG' value <-25 kJ mol(-1) was reached. Benzoyl-CoA reductase was not detected. Enzyme activities for all reaction steps from glutaryl-CoA to butyryl-CoA, and ATP formation via acetate kinase were detected in cell-free extracts. Glutaconyl-CoA decarboxylase is likely to act as a primary sodium ion pump.
引用
收藏
页码:288 / 295
页数:8
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