Excision-repair patch lengths are similar for transcription-coupled repair and global genome repair in UV-irradiated human cells

被引：22

作者：

Bowman, KK ^{[1
]}

Smith, CA ^{[1
]}

Hanawalt, PC ^{[1
]}

机构：

[1] Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA

来源：

MUTATION RESEARCH-DNA REPAIR | 1997年 / 385卷 / 02期

关键词：

DNA repair; transcription; transcription coupled repair; global genome repair; xeroderma pigmentosum; complementation group C; XPC; human; fibroblast; HT1080; XP10BE; patch; density shift; alkaline CsCl gradient; bromodeoxyuridine;

D O I：

10.1016/S0921-8777(97)00029-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have used the buoyant density shift method to measure excision-repair patch lengths in W-irradiated repair-proficient human cells and in primary fibroblasts belonging to xeroderma pigmentosum complementation group C (XP-C), in which excision repair of UV-induced photoproducts is dependent upon transcription. The patch size was found to be about 30 nucleotides for both cell types. This agrees with the size of the DNA fragments excised in vitro by the dual incisions of the structure-specific nucleases XPG and ERCC1-XPF. We conclude that the XPC protein is not required to target the excision nucleases to sites of DNA cleavage in transcribed strands of expressed genes or to protect the newly incised DNA from further processing by exonucleases. (C) 1997 Elsevier Science B.V.

引用

页码：95 / 105

页数：11

共 54 条

[1] MAMMALIAN DNA NUCLEOTIDE EXCISION-REPAIR RECONSTITUTED WITH PURIFIED PROTEIN-COMPONENTS [J].