Tracking dendritic cells:: use of an in situ method to label all blood leukocytes

被引：16

作者：

Ristevski, B

Young, AJ

Dudler, L

Cahill, RNP

Kimpton, W

Washington, E

Hay, JB

机构：

[1] Univ Toronto, Dept Lab Med & Pathol, Toronto, ON M5S 1A8, Canada

[2] Basel Inst Immunol, CH-4005 Basel, Switzerland

[3] Univ Melbourne, Lab Foetal & Neonatal Immunol, Parkville, Vic 3052, Australia

[4] Univ Toronto, Dept Immunol, Toronto, ON M5S 1A8, Canada

来源：

INTERNATIONAL IMMUNOLOGY | 2003年 / 15卷 / 02期

关键词：

carboxyfluorescein diacetate succinimidyl ester; lymph; lymph node; recirculation;

D O I：

10.1093/intimm/dxg016

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Here we describe an in situ procedure with a labeling index (percent of labeled blood leukocytes) >98%, which is high enough to permit the direct tracking of dendritic cell (DC) precursors from blood into lymphoid tissues, while circumventing the pitfalls associated with in vitro labeling. DC and lymphocytes have similar blood to afferent lymph migratory capabilities. This method has additional applications in tracking other rare cell populations in both normal and pathological states.

引用

页码：159 / 165

页数：7

共 30 条

[1] In vivo labeling of neutrophils using a fluorescent cell linker [J].