Induction and verification of autotetraploids in diploid banana (Musa acuminata) by in vitro techniques

被引:94
作者
vanDuren, M [1 ]
Morpurgo, R [1 ]
Dolezel, J [1 ]
Afza, R [1 ]
机构
[1] DE MONTFORT UNIV, NORMAN BORLAUG CTR PLANT SCI, INST EXPT BOT, CR-77200 OLOMOUC, CZECH REPUBLIC
关键词
banana breeding; chimerism; colchicine; flow cytometry; mixoploidy; Musa; oryzalin; tetraploids;
D O I
10.1007/BF00029262
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Flow cytometry and stomata characteristics were used for screening ploidy levels in a large population of in vitro induced autopolyploids of the Musa acuminata breeding clone SH-3362. Culturing shoot tips in liquid medium supplemented both with 5.0 mM colchicine for 48 hours or 30 mu M oryzalin (3,5-dinitro-N4,N-dipropylsulphate) for seven days, both in combination with 2% (v/v) DMSO, resulted in a high (23.1% and 29.1%) frequency of non-chimeric tetraploids in the fourth vegetative generation. Although mixoploidy persisted in subsequent cycles of vegetative propagation, tetraploids as identified by flow cytometry remained solid non-chimeric during two more cycles. These autotetraploids were propagated for field testing. A rough pre-selection of regenerated V4 plants based on their stomata characteristics resulted in a population in which only 56.2% of the plants were solid tetraploids. The somatic polyploidization system reported here can be utilised for banana breeding programmes.
引用
收藏
页码:25 / 34
页数:10
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