A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs

被引：88

作者：

Lam, TL ^{[1
]}

Lam, ML ^{[1
]}

Au, TK ^{[1
]}

Ip, DTM ^{[1
]}

Ng, TB ^{[1
]}

Fong, WP ^{[1
]}

Wan, DCC ^{[1
]}

机构：

[1] City Univ Hong Kong, Dept Biochem, Shatin, Hong Kong, Peoples R China

来源：

LIFE SCIENCES | 2000年 / 67卷 / 23期

关键词：

Chinese medicinal herbs; HIV-1; protease; fluorogenic assay; HPLC assay;

D O I：

10.1016/S0024-3205(00)00864-X

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The aqueous and methanol extracts of thirty-one herbs traditionally used as anti-fever remedies in China were screened for their in vitro inhibition on human immunodeficiency virus type-1 protease (HIV-1 PR). The activity of recombinant HIV-1 protease was determined by sequence-specific cleavage at the Tyr-Pro bond of the fluorogenic substrate (Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(DABCYL)-Arg) or by HPLC anaylsis of the cleavage products after incubation of the enzyme with a synthetic peptide substrate (Acetyl-Ser-Gln-Asn-Tyr-Pro-Val-Val-amide) Among the herbal extracts examined, the aqueous extracts of Prunella vulgaris and Scutellaria baicalensis and the methanol extracts of Woodwardia unigemmata, Paeonica suffruticosa and Spatholobus suberectus elicited significant inhibition (>90%) at a concentration of 200 mug/ml. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：2889 / 2896

页数：8

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