Micrografting of mature stone pine (Pinus pinea L.) trees

被引:10
作者
Cortizo, M
Alonso, P
Fernández, B
Rodríguez, A
Centeno, ML
Ordás, RJ
机构
[1] Univ Oviedo, Dept Biol Organismos & Sistemas, Unidad Fisiol Vegetal, E-33071 Oviedo, Spain
[2] Univ Leon, Dept Biol Vegetal, E-24071 Leon, Spain
关键词
micro-greffage; micro-propagation; conifer; in vitro greffage; Pinus pinea;
D O I
10.1051/forest:2004081
中图分类号
S7 [林业];
学科分类号
0829 ; 0907 ;
摘要
This paper describes an in vitro micrografting method for selected mature Pinus pinea L. trees. Needle fascicles of five selected clones were micrografted onto hypocotyls of two weeks-old germinated isolated embryos. Fascicle meristems outgrowth was recorded after one month of culture and the performance of the clones assayed was evaluated. Clones behave statistically different in establishment and development rates. Overall success of our protocol reached 43% of the graftings made.
引用
收藏
页码:843 / 845
页数:3
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