Human ORFeome version 1.1: A platform for reverse proteomics

被引：170

作者：

Rual, JF

Hirozane-Kishikawa, T

Hao, T

Bertin, N

Li, SM

Dricot, A

Li, N

Rosenberg, J

Lamesch, P

Vidalain, PO

Clingingsmith, TR

Hartley, JL

Esposito, D

Cheo, D

Moore, T

Simmons, B

Sequerra, R

Bosak, S

Doucette-Stamm, L

Le Peuch, C

Vandenhaute, J

Cusick, ME

Albala, JS

Hill, DE ^{[1
]}

Vidal, M

机构：

[1] Harvard Univ, Sch Med, Dana Farber Canc Inst, Ctr Canc Syst Biol, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

[4] Fac Univ Notre Dame Paix, B-5000 Namur, Belgium

[5] CNRS, FRE 2593, Ctr Rech Biochim Macromol, F-34293 Montpellier 5, France

[6] NCI, Sci Applicat Int Corp, Frederick, MD 21702 USA

[7] Atto Biosci, Rockville, MD 20850 USA

[8] Open Biosyst Inc, Huntsville, AL 35806 USA

[9] Agencourt Biosci Corp, Beverly, MA 01905 USA

[10] Lawrence Livermore Natl Lab, Biol & Biotechnol Res Program, Livermore, CA 94551 USA

来源：

GENOME RESEARCH | 2004年 / 14卷 / 10B期

关键词：

D O I：

10.1101/gr.2973604

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The advent of systems biology necessitates the cloning of nearly entire sets of protein-encoding open reading frames (ORFs), or ORFeomes, to allow functional studies of the corresponding proteomes. Here, we describe the generation of a first version of the human ORFeome using a newly improved Gateway recombinational cloning approach. Using the Mammalian Gene Collection (MGC) resource as a starting point, we report the successful cloning of 8076 human ORFs, representing at least 7263 human genes, as mini-pools of PCR-amplified products. These were assembled into the human ORFeome version 1.1 (hORFeome v1.1) collection. After assessing the overall quality of this version, we describe the use of hORFeome v1.1 for heterologous protein expression in two different expression systems at proteome scale. The hORFeome v1.1 represents a central resource for the cloning of large sets of human ORFs in various settings for functional proteomics of many types, and will serve as the foundation for subsequent improved versions of the human ORFeome.

引用

页码：2128 / 2135

页数：8

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