Expression of transforming growth factors alpha and beta-1 messenger RNA in the bovine mammary gland during different stages of development and lactation

被引:63
作者
Plath, A
Einspanier, R
Peters, F
Sinowatz, F
Schams, D
机构
[1] TECH UNIV MUNCHEN WEIHENSTEPHAN,INST PHYSIOL,D-85350 FREISING,GERMANY
[2] ST HILDEGARDIS HOSP,D-55128 MAINZ,GERMANY
[3] LMU MUNICH,D-80539 MUNICH,GERMANY
关键词
D O I
10.1677/joe.0.1550501
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
It is now widely accepted that the mammary gland is under interconnected hormonal and local control. Growth factors are involved in the intercellular signalling of the gland. Our aim was the detection of transforming growth factors alpha (TGF-alpha) and beta 1 (TGF-beta 1) messenger RNA during mammogenesis, lactogenesis, galactopoiesis and involution in the bovine mammary gland (total n=27). During these stages the RNA was assessed by means of ribonuclease protection assay and reverse transcription-polymerase chain reaction (RT-PCR). To study possible influences of oestrogen, progesterone and prolactin on growth factor expression, mammary RNA was obtained from heifers after induced mammogenesis and lactogenesis, with and without additional prolactin inhibition (total n=20). Very low levels of TGF-alpha and TGF-beta 1 expression were detected during lactogenesis and galactopoiesis, increasing levels during mammogenesis of primigravid heifers, and highest levels during mammo-genesis of virgin heifers and during involution. TGF-alpha expression after induced mammogenesis was greater than after induced lactogenesis or physiological mammogenesis during pregnancy. Furthermore, TGF-alpha mRNA contents increased after prolactin inhibition. TGF-beta 1 expression was almost equal after induced mammogenesis and lactogenesis, but greater than during the physiological mammogenesis and lactogenesis. In conclusion, it can be assumed that growth promoting TGF-alpha and growth inhibiting TGF-beta 1 are co-expressed in the bovine mammary gland. Higher mRNA contents of both factors during mammogenesis and involution may indicate autocrine or paracrine functions for these growth factors during proliferation and reorganisation of the mammary tissue.
引用
收藏
页码:501 / 511
页数:11
相关论文
共 51 条
[1]   THE CELL BIOLOGY OF TRANSFORMING GROWTH-FACTOR-BETA [J].
BARNARD, JA ;
LYONS, RM ;
MOSES, HL .
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1032 (01) :79-87
[2]   TRANSCRIPTS FOR TRANSFORMING GROWTH-FACTORS IN HUMAN BREAST-CANCER - CLINICAL CORRELATES [J].
BARRETTLEE, P ;
TRAVERS, M ;
LUQMANI, Y ;
COOMBES, RC .
BRITISH JOURNAL OF CANCER, 1990, 61 (04) :612-617
[3]   EXPRESSION OF THE TRANSFORMING GROWTH FACTOR-ALPHA EPIDERMAL GROWTH-FACTOR RECEPTOR PATHWAY IN NORMAL HUMAN-BREAST EPITHELIAL-CELLS [J].
BATES, SE ;
VALVERIUS, EM ;
ENNIS, BW ;
BRONZERT, DA ;
SHERIDAN, JP ;
STAMPFER, MR ;
MENDELSOHN, J ;
LIPPMAN, ME ;
DICKSON, RB .
ENDOCRINOLOGY, 1990, 126 (01) :596-607
[4]  
Bissell M. J., 1987, The mammary gland. Development, regulation, and function., P97
[5]   TRANSMEMBRANE TGF-ALPHA PRECURSORS ACTIVATE EGF TGF-ALPHA RECEPTORS [J].
BRACHMANN, R ;
LINDQUIST, PB ;
NAGASHIMA, M ;
KOHR, W ;
LIPARI, T ;
NAPIER, M ;
DERYNCK, R .
CELL, 1989, 56 (04) :691-700
[6]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[7]   MILK-PRODUCTION AND REPRODUCTIVE-PERFORMANCE OF COWS HORMONALLY INDUCED INTO LACTATION [J].
COLLIER, RJ ;
BAUMAN, DE ;
HAYS, RL .
JOURNAL OF DAIRY SCIENCE, 1975, 58 (10) :1524-1527
[8]   REGULATION OF BOVINE MAMMARY GROWTH BY PEPTIDE-HORMONES - INVOLVEMENT OF RECEPTORS, GROWTH-FACTORS AND BINDING-PROTEINS [J].
COLLIER, RJ ;
MCGRATH, MF ;
BYATT, JC ;
ZURFLUH, LL .
LIVESTOCK PRODUCTION SCIENCE, 1993, 35 (1-2) :21-33
[9]   ISOLATION AND CHARACTERIZATION OF MILK GROWTH-FACTOR, A TRANSFORMING-GROWTH-FACTOR-BETA-2-RELATED POLYPEPTIDE, FROM BOVINE-MILK [J].
COX, DA ;
BURK, RR .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 197 (02) :353-358
[10]  
DEOME KB, 1959, CANCER RES, V19, P515