Structural characterization of immunostimulating polysaccharide from cultured mycelia of Cordyceps militaris

被引：148

作者：

Lee, Jong Seok ^{[1
]}

Kwon, Jeong Seok ^{[1
]}

Yun, Jong Seok ^{[1
]}

Pahk, Jung Woon ^{[1
]}

Shin, Won Cheol ^{[1
]}

Lee, Shin Young ^{[1
]}

Hong, Eock Kee ^{[1
]}

机构：

[1] Kangwon Natl Univ, Dept Bioengn & Technol, Coll Engn, Chunchon 200701, Gangwon Do, South Korea

来源：

CARBOHYDRATE POLYMERS | 2010年 / 80卷 / 04期

关键词：

Cordyceps militaris; Immunostimulating polysaccharide; Macrophage activation; Random coil conformation; ANTITUMOR-ACTIVITY; MOLECULAR-WEIGHT; FRUITING BODIES; BETA-GLUCANS; SINENSIS; (1->3)-BETA-D-GLUCAN; ELUCIDATION; MECHANISMS; INDUCTION; APOPTOSIS;

D O I：

10.1016/j.carbpol.2010.01.017

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

The water soluble crude polysaccharide obtained from cultured mycelia of Cordyceps militaris (CPM) by hot water extraction followed by ethanol precipitation was fractionated by DEAE cellulose and Sepharose CL-6B column chromatography. This fractionation process resulted in four polysaccharide fractions that were termed CPMN Fr I, CPMN Fr II, CPMN Fr III, and CPMN Fr IV. Of these fractions, CPMN Fr III was able to upregulate the functional events mediated by activated macrophages, such as production of nitric oxide (NO) and expression of cytokines (IL-1 beta and TNF-alpha). Its structural characteristics were investigated by a combination of chemical and instrumental analyses, including methylation, reductive cleavage, acetylation, Fourier transform infrared spectroscopy (FT-IR), and gas chromatography-mass spectrometry (GC-MS). Results indicate that CPMN Fr III was a high molecular mass polysaccharide with a random coil conformation of the beta-1,4-branched-beta-1,6-galactoglucomannan. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1011 / 1017

页数：7

共 42 条

[1]

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