Mapping and validation of quantitative trait loci for spikelets per panicle and 1,000-grain weight in rice (Oryza sativa L.)

This study identified four and five quantitative trait loci (QTLs) for 1,000-grain weight (TGW) and spikelets per panicle (SPP), respectively, using rice recombinant inbred lines. QTLs for the two traits (SPP3a and TGW3a, TGW3b and SPP3b) were simultaneously identified in the two intervals between RM3400 and RM3646 and RM3436 and RM5995 on chromosome 3. To validate QTLs in the interval between RM3436 and RM5995, a BC3F2 population was obtained, in which TGW3b and SPP3b were simultaneously mapped to a 2.6-cM interval between RM15885 and W3D16. TGW3b explained 50.4% of the phenotypic variance with an additive effect of 1.81 g. SPP3b explained 29.1% of the phenotypic variance with an additive effect of 11.89 spikelets. The interval had no effect on grain yield because it increased SPP but decreased TGW and vice versa. Grain shape was strongly associated with TGW and was used for QTL analysis in the BC3F2 population. Grain length, grain width, and grain thickness were also largely controlled by TGW3b. At present, it is not clear whether one pleiotropic QTL or two linked QTLs were located in the interval. However, the conclusion could be made ultimately by isolation of TGW3b. The strategy for TGW3b isolation is discussed.