Molecular determinants of HIV-1 intersubtype recombination potential

被引：33

作者：

Chin, Mario P. S. ^{[1
]}

Chen, Jianbo ^{[1
]}

Nikolaitchik, Olga A. ^{[1
]}

Hu, Wei-Shau ^{[1
]}

机构：

[1] Natl Canc Inst, HIV Drug Resistance Program, Frederick, MD 21702 USA

来源：

VIROLOGY | 2007年 / 363卷 / 02期

关键词：

HIV-1; subtype; recombination rate; dimerization initiation signal; heterozygous virus; RNA packaging;

D O I：

10.1016/j.virol.2007.01.034

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sequence differences in the dimerization initiation signal (DIS) affect the rate of recombination between subtype B and subtype C HIV-1. To test the hypothesis that DIS sequences can be used to predict intersubtype recombination potentials, we measured the recombination rate between CRF01_A/E (AE) and B, which contain mismatches in the DIS, and between AE and C, which have an identical DIS. Compared with the intrasubtype recombination rate, the recombination rate between AE and subtype B virus was 9-fold lower, and the rate between AE and subtype C virus was 2-fold lower. Thus, DIS sequences can be used to predict the recombination potential between HIV-1 subtypes. Further analyses revealed that the 2-fold lower recombination rate between AE and C viruses can be restored to the intrasubtype recombination rate by matching a part of the LTR and a portion of the viral genome. Therefore, the lower intersubtype recombination rate between AE and C is not caused by a given region but is a cumulative effect by more than one region. Published by Elsevier Inc.

引用

页码：437 / 446

页数：10

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