Biodiversity of chloramphenicol-resistant mesophilic heterotrophs from Southeast Asian aquaculture environments

被引:61
作者
Huys, Geert [1 ]
Bartie, Kerry
Cnockaert, Margo
Oanh, Dang Thi Hoang
Phuong, Nguyen Thanh
Somsiri, Temdoung
Chinabut, Supranee
Yusoff, Fatimah Md
Shariff, Mohamed
Giacomini, Mauro
Teale, Alan
Swings, Jean
机构
[1] Univ Ghent, Microbiol Lab, B-9000 Ghent, Belgium
[2] Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland
[3] Can Tho Univ, Lab Fish Dis, Coll Aquaculture & Fisheries, Can Tho City, Vietnam
[4] Aquat Anim Hlth Res Inst, Bangkok 10900, Thailand
[5] Univ Pertanian Malaysia, Inst Biosci, Serdang 43400, Selangor, Malaysia
[6] Univ Genoa, DIST, Genoa, Italy
[7] Univ Ghent, BCCM, LMG Bacteria Collect, B-9000 Ghent, Belgium
关键词
taxonomy; antibiotic resistance; human opportunists;
D O I
10.1016/j.resmic.2006.12.011
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In the present study, samples of pond water, sediment and farmed species were collected at 12 fish and shrimp farms in Malaysia, Thailand and Vietnam to determine the biodiversity and environmental distribution of chloramphenicol-resistant (CmR) mesophilic heterotrophs in Southeast Asian aquaculture sites. Following isolation on Iso-Sensitest agar supplemented with 35 mu g ml(-1) Cm and dereplication using (GTG)(5)-PCR fingerprinting, 557 genotypically unique CmR strains were subjected to polyphasic identification. The 557 mesophilic heterotrophic CmR isolates represented 18 different genera largely dominated by the genera Escherichia (40.2%), Pseudomonas (11.7%), Acinetobacter (11. 1 %), Klebsiella (7.5%) and Bacillus (5.9%). A total of 439 CmR isolates were further assigned to 31 described species or species groups, mainly including organisms that have been associated with various human opportunistic infections such as Escherichia coli (n = 219), Pseudomonas putida (n = 47), Klebsiella pneumoniae (n = 38) and Acinetobacter baumannii (n = 23). Strains of Escherichia, and most notably, of E. coli, were the only common group of CmR heterotrophs irrespective of country, sample type or farm type. Together with other predominant but less widespread groups such as acinetobacters and pseudomonads, the results of this biodiversity study suggest that E. coli can be regarded as a potential indicator of Cm resistance in Southeast Asian aquaculture environments. (c) 2007 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:228 / 235
页数:8
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