The rice metallothionein gene promoter does not direct foreign gene expression in seed endosperm

被引:17
作者
Fukuzawa, H
Yu, LH
Umeda-Hara, C
Tagawa, M
Uchimiya, H
机构
[1] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan
[2] Nissan Chem Ind Co Ltd, Shiraoka Res Stn Biol Sci, Dept Agr Chem, Minami Saitama, Saitama 3490294, Japan
关键词
endosperm; gene promoter; metallothionein; rice; tobacco;
D O I
10.1007/s00299-004-0813-z
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We generated transgenic tobacco and rice plants harboring a chimeric gene consisting of the 5'-upstream sequence of the rice metallothionein gene (ricMT) fused to the beta-glucuronidase (GUS) gene. The activity and tissue-specific expression of the ricMT promoter were demonstrated in these transgenic plants. In the transgenic rice plants, despite substantial levels of GUS activity in the shoot and root, almost no GUS signal was detected in the endosperm. Thus, the ricMT promoter could be useful in avoiding accumulation of undesired proteins in the seed endosperm.
引用
收藏
页码:231 / 235
页数:5
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