28-norcastasterone is biosynthesized from castasterone

被引：23

作者：

Fujioka, S ^{[1
]}

Noguchi, T

Sekimoto, M

Takatsuto, S

Yoshida, S

机构：

[1] RIKEN, Inst Phys & Chem Res, Wako, Saitama 3510198, Japan

[2] Tama Biochem Co Ltd, Shinjuku Ku, Tokyo 1630704, Japan

[3] Joetsu Univ Educ, Dept Chem, Joetsu, Niigata 9438512, Japan

来源：

PHYTOCHEMISTRY | 2000年 / 55卷 / 02期

关键词：

Arabidopsis thaliana; Carharanthus roseus; Apocynaceae; Cruciferae; biosynthesis; cultured cells; biosynthetic intermediates of brassinolide; castasterone; 28-norcastasterone; 28-nortyphasterol;

D O I：

10.1016/S0031-9422(00)00261-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Metabolic experiments with deuterium-labeled castasterone in seedlings of Arabidopsis thaliana, Oryza sativa and Lycopersicon esculentum, and cultured cells of Catharanthus roseus were performed, and the metabolites were analyzed by GC-MS. In all the plans species examined. [H-2(3)]28-norcastasterone was identified as a metabolite of [26,28-H-2(6)]castasterone, indicating that castasterone is the biosynthetic origin of 28-norcastasterone. Moreover, the natural occurrence of 28-norcastasterone and 28-nortyphasterol in seedlings of A. thaliana has been demonstrated. This is the first report of the natural occurrence of 28-nortyphasterol in plants. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：97 / 101

页数：5

共 15 条

[1] Recent advances in brassinosteroid molecular genetics [J].

Altmann, T .

CURRENT OPINION IN PLANT BIOLOGY, 1998, 1 (05) :378-383

[2]

Clouse SD, 1999, BRASSINOSTEROIDS, P163

[3] Brassinosteroids [J].

Fujioka, S ;

Sakurai, A .

NATURAL PRODUCT REPORTS, 1997, 14 (01) :1-10

[4]

Fujioka S, 1999, BRASSINOSTEROIDS, P21

[5] Biosynthesis of brassinosteroids in cultured cells of Catharanthus roseus [J].

Fujioka, S ;

Noguchi, T ;

Watanabe, T ;

Takatsuto, S ;

Yoshida, S .

PHYTOCHEMISTRY, 2000, 53 (05) :549-553

[6] Brassinosteroids in Arabidopsis thaliana [J].

Fujioka, S ;

Noguchi, T ;

Yokota, T ;

Takatsuto, S ;

Yoshida, S .

PHYTOCHEMISTRY, 1998, 48 (04) :595-599

[7] Biosynthesis and metabolism of brassinosteroids [J].

Fujioka, S ;

Sakurai, A .

PHYSIOLOGIA PLANTARUM, 1997, 100 (03) :710-715

[8] The Arabidopsis deetiolated2 mutant is blocked early in Brassinosteroid biosynthesis [J].

Fujioka, S ;

Li, JM ;

Choi, YH ;

Seto, H ;

Takatsuto, S ;

Noguchi, T ;

Watanabe, T ;

Kuriyama, H ;

Yokota, T ;

Chory, J ;

Sakurai, A .

PLANT CELL, 1997, 9 (11) :1951-1962

[9] Activity of brassinosteroids in the dwarf rice lamina inclination bioassay [J].

Fujioka, S ;

Noguchi, T ;

Takatsuto, S ;

Yoshida, S .

PHYTOCHEMISTRY, 1998, 49 (07) :1841-1848

[10] Brassinosteroid-insensitive dwarf mutants of Arabidopsis accumulate brassinosteroids [J].

Noguchi, T ;

Fujioka, S ;

Choe, S ;

Takatsuto, S ;

Yoshida, S ;

Yuan, H ;

Feldmann, KA ;

Tax, FE .

PLANT PHYSIOLOGY, 1999, 121 (03) :743-752

← 1 2 →