Sensitive and semi-quantitative TaqMan™ real-time polymerase chain reaction systems for the detection of beef (Bos taurus) and the detection of the family Mammalia in food and feed

被引：109

作者：

Brodmann, PD

Moore, D

机构：

[1] Kantonales Lab Basel Stadt, CH-4056 Basel, Switzerland

[2] Bundesamt Gesundheit, CH-3000 Bern 14, Switzerland

来源：

MEAT SCIENCE | 2003年 / 65卷 / 01期

关键词：

real-time PCR; bovine growth hormone gene; meat and bone meal; food; Bos taurus;

D O I：

10.1016/S0309-1740(02)00253-X

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Consumers distrust beef or products that could contain beef because of the BSE (bovine spongiform encephalopathy) and vCJD (variant Creutzfeld Jacob Disease) cases during recent years. Cows could be fed with meat and bone meal-containing food. To regain consumer confidence methods are needed that allow the detection of smallest amounts of beef in the most different kind of products. Polymerase chain reaction (PCR) analysis can be used to detect the smallest amounts of even highly degraded DNA. In this work two methods are presented that allow the detection of mammal DNA and beef DNA, respectively, even in highly degraded DNA. The amplification of fragments as short as 66 and 76 bp, respectively, allow a determination of mammal or beef DNA even in meat and bone meal products. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：599 / 607

页数：9

共 39 条

[1] Fears rise over BSE infection in UK abattoirs [J].