Molecular cloning and gene expression of growth hormone-releasing peptide receptor in rat tissues

被引：66

作者：

Yokote, R

Sato, M

Matsubara, S

Ohye, H

Niimi, M

Murao, K

Takahara, J

机构：

[1] Kagawa Med Univ, Dept Internal Med 1, Kagawa, Japan

[2] Kagawa Med Univ, Dept Clin Lab Med, Kagawa, Japan

来源：

PEPTIDES | 1998年 / 19卷 / 01期

关键词：

growth hormone-releasing peptide; receptor; rat; gene; polymerase chain reaction; hypothalamus; renal pelvis;

D O I：

10.1016/S0196-9781(97)00263-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We cloned a fragment of the rat GH-releasing peptide (GHRP) receptor homologue and examined the tissue distribution of GHRP receptor mRNA in rats. Sequence analysis showed that the open reading frame is well conserved between rat and human with 96% identity in a 364-amino acid overlap. By reverse transcription-polymerase chain reaction we detected GHRP receptor mRNAs in the rat brain including the hypothalamus, anterior pituitary, and renal pelvis in twenty-eight tissues tested. Microdissection revealed that GHRP receptor mRNAs were localized predominantly in the arcuate nucleus and ventromedial hypothalamus. (C) 1998 Elsevier Science Inc.

引用

页码：15 / 20

页数：6

共 34 条

[1] THE GROWTH-HORMONE SECRETAGOGUE, L-692,429, INDUCES PHOSPHATIDYLINOSITOL HYDROLYSIS AND HORMONE-SECRETION BY HUMAN PITUITARY-TUMORS [J].